Screening and Identification Strategies of Aptamers for Small Molecules
10.13865/j.cnki.cjbmb.2025.09.1209
- VernacularTitle:小分子靶标适配体的筛选与鉴定策略
- Author:
Xiao-Juan ZHANG
1
;
Liang-Hua WANG
Author Information
1. 绍兴文理学院医学实验中心,浙江绍兴 312000
- Publication Type:Journal Article
- Keywords:
small molecule;
aptamer;
systematic evolution of ligands by exponential enrichment(SEL-EX);
structural analysis;
affinity determination
- From:
Chinese Journal of Biochemistry and Molecular Biology
2025;41(11):1656-1667
- CountryChina
- Language:Chinese
-
Abstract:
Small molecules generally refer to natural compounds with a molecular weight less than 1 kD,which have significant research value in many fields such as biological science,pharmaceutical and health care,and environmental science,etc.Aptamers,as a new type of bio-recognition element,can bind specifically to small molecules and other targets with high affinity.They are single-stranded short nu-cleotide sequences selected by the systematic evolution of ligands by exponential enrichment(SELEX)technology.Compared with aptamers of large molecules,aptamers of small molecules often exhibit disad-vantages such as difficult screening,low affinity,and poor specificity.To solve these bottleneck prob-lems,the article provides some strategies of screening and identification of aptamers for small molecules.Firstly,when designing the screening plan for aptamers of small molecules,this article suggests focusing on key points such as library design,SELEX methods,amplification methods,screening process monito-ring methods,and aptamer sequence analysis methods,then choosing or designing appropriate strategies based on the actual situation.Secondly,the structure and affinity of aptamers directly affect their applica-tions.The article briefly introduces methods for analyzing the secondary and tertiary structures of aptam-ers and six common methods for affinity determination.Finally,the article discusses some problems exist-ing in screening and identification of aptamers for small molecules and looks forward to future develop-ment prospects.The whole article aims to provide research strategies for multiple steps of screening and i-dentification of aptamers for small molecules and offer references for improving the efficiency of aptamer screening and identification.
