Single-cell Sequencing and Spatial Transcriptome Sequencing:Unveiling the Heterogeneity of Mesenchymal Stem Cells and the Dynamic Evolution of the Spatial Microenvironment
10.13865/j.cnki.cjbmb.2025.10.1268
- VernacularTitle:单细胞测序与空间转录物组测序:揭秘间充质干细胞异质性与空间微环境的动态演变
- Author:
Liu-Jia-Yu LI
1
;
Cheng WANG
;
Li-Mei YU
Author Information
1. 遵义医科大学附属医院贵州省生物制造实验室,贵州遵义 563000;遵义医科大学附属医院贵州省细胞工程重点实验室,贵州遵义 563000;遵义医科大学教育部组织损伤修复与再生医学省部共建协同创新中心,贵州遵义 563006
- Publication Type:Journal Article
- Keywords:
single-cell transcriptome sequencing(scRNA-seq);
spatial transcriptomics(ST-seq);
mesenchymal stem cells(MSCs);
regenerative medicine
- From:
Chinese Journal of Biochemistry and Molecular Biology
2025;41(11):1610-1621
- CountryChina
- Language:Chinese
-
Abstract:
Mesenchymal stem cells(MSCs)hold great promise in regenerative medicine due to their multi-lineage differentiation potential and immunomodulatory properties.However,their functional heter-ogeneity and strong dependency on the microenvironment remain major challenges for clinical application.In recent years,the combination of single-cell transcriptome sequencing(scRNA-seq)and spatial tran-scriptomics sequencing(ST-seq)has provided revolutionary tools for systematically deciphering the heter-ogeneity,functional diversity,and microenvironmental interactions of MSCs.Using scRNA-seq,re-searchers have successfully resolved the functional heterogeneity of MSCs and identified key functional subpopulations,such as pro-angiogenic,immunoregulatory,and matrix-remodeling subsets.Meanwhile,ST-seq has revealed the distinct spatial distribution of MSCs within tissues and their dynamic interaction networks with the microenvironment.The integration of these two technologies has not only enabled the construction of a three-dimensional"identity-location-function"atlas of MSCs,but also uncovered spatio-temporal dynamic regulatory mechanisms of specific subpopulations during tissue repair.Looking forward,the combination of ultra-high-resolution ST-seq platforms such as Xenium,multi-omics integration,and artificial intelligence-driven analysis will shift MSCs research from descriptive studies toward precise inter-vention,offering new strategies for functional subpopulation screening and microenvironment reprogram-ming therapy.This review systematically summarizes the latest advances in scRNA-seq and ST-seq tech-nologies in MSC research,discusses their applications in elucidating cellular heterogeneity,spatial micro-environment,and clinical translation,and provides a theoretical basis and technical guidance for preci-sion treatment in regenerative medicine.
