Nanoplastics and microplastics impair spatial memory ability in mice by inhibiting autophagy
10.16303/j.cnki.1005-4545.2025.10.20
- VernacularTitle:纳米塑料和微塑料通过抑制自噬损伤小鼠空间记忆能力
- Author:
Huimei LIANG
1
;
Jiarui PAN
1
;
Xueer LIN
1
;
Minyi ZHAO
1
;
Huan ZENG
1
;
Yuqiang CHEN
1
;
Hou-hui SONG
1
;
Wei WANG
1
;
Jinghua ZHAO
1
Author Information
1. 浙江农林大学动物医学院浙江省畜禽绿色生态健康养殖应用技术研究重点实验室/动物健康互联网检测技术浙江省工程研究中心/浙江省动物医学与健康管理国际科技合作基地/同一健康和食品安全一带一路国际联合实验室/中澳动物健康大数据分析联合实验室,浙江 杭州 311300
- Publication Type:Journal Article
- Keywords:
nanoplastics;
microplastics;
autophagy;
glutamate receptor;
spatial memory;
mice
- From:
Chinese Journal of Veterinary Science
2025;45(10):2246-2255
- CountryChina
- Language:Chinese
-
Abstract:
Approximately 300 million tons of plastic are produced globally each year,which has a serious impact on human health,marine life and the livestock industry.Microplastics have also been detected in meat and milk samples.Research has shown that nanoplastics(NP)(＜1 μm)and mi-croplastics(MP)(1 μm-5 mm)can affect the digestive,immune and reproductive systems of ani-mals.This experiment aims to investigate whether NP and MP regulate autophagy and damage the nervous system and spatial memory of animals.This experiment was divided into control group,nanoplastic group(PS-NP group,0.1 μm)and microplastic group(PS-MP group,1 μm),with 20 mice in each group.The mice were given 0.5 mL of PS-NP and PS-MP every day for 35 consecutive days,followed by neck amputation and brain analysis.The results showed that NPs and MPs of dif-ferent diameters caused varying degrees of damage to the brains of mice.In the behavioral tests of new object recognition,barnes maze and Y-shaped maze spatial memory,compared with the control group,the PS-NP group and PS-MP group showed a significant decrease in spatial memory ability of mice.HE staining results showed that neuronal cells in the PS-NP and PS-MP groups of mice exhibited shrinkage,decreased cell volume and deepened staining.The number of Nissl bodies de-creased,leading to dissolution and disappearance.RT-PCR and Western blot results showed that compared with the control group,the expression of glutamate receptors NR1,NR2A and NR2B in-creased in mice administered NP and MP orally,while the expression of autophagy related proteins Parkin,LC3B and Beclin1 was inhibited.In summary,this study suggests that nanoplastics and mi-croplastics stimulate glutamate receptors in mice by inhibiting the autophagy pathway,leading to impaired spatial memory.
