Effects and mechanisms of the aqueous extract of Artemisia capillaris(Yin Chen)in alleviating liver injury induced by LPS combined with D-GalN in mice
10.16303/j.cnki.1005-4545.2025.10.19
- VernacularTitle:茵陈水提液缓解LPS联合D-GalN致小鼠肝脏损伤的作用与机制
- Author:
Yanan CHUO
1
;
Jiaxue SHEN
;
Weiyu YAO
;
Xiao WANG
;
Yongzhan BAO
;
Jinxu TANG
;
Wanyu SHI
Author Information
1. 河北农业大学中兽医学院,河北保定 071001
- Publication Type:Journal Article
- Keywords:
the aqueous extract of Yin Chen;
liver injury;
IL-17/TNF pathway;
inflammatory cyto-kines;
oxidative stress;
mice
- From:
Chinese Journal of Veterinary Science
2025;45(10):2239-2245,2263
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the mitigating effect of Yin Chen aqueous extract on liver injury induced by LPS combined with D-GalN in mice,a mouse liver injury model was established by in-traperitoneal injection of LPS and D-GalN,and the mice were group-fed by instillation of saline,bi-phenyl dibenzyl ester,and Yin Chen aqueous extract with different concentrations of LPS and D-GalN.The liver index of mice was calculated,pathological tissue sections were observed,and the expression of ALT,AST,inflammatory cytokines,oxidative stress,hepatic enzymes,and IL-17/TNF pathway were detected in serum to investigate the effects and mechanisms of the aqueous ex-tracts of Yin Chen in alleviating the liver injury in mice.The results showed that the liver index of mice in the model group was significantly elevated,the serum levels of ALT and AST were signifi-cantly elevated,the levels of IL-iβ,IL-8 and TNF-α in liver tissue were significantly elevated,the levels of IL-4 were significantly reduced,the levels of GSH-Px,CAT and SOD were significantly reduced,the levels of ROS and MDA were significantly elevated,CYP2E1 and CYP1A2 protein content and mRNA expression were significantly up-regulated,and the expression levels of TNF,TNFR1,IL-17A,ACT1 and IL-6 mRNA were significantly up-regulated.The study showed that the aqueous extract of Yin Chen had a certain alleviating effect on the liver injury caused by LPS combined with D-GalN in mice.The mechanism of action includes decreasing the metabolic level of hepatic drug enzymes,alleviating oxidative stress,inhibiting the expression of IL-17/TNF pathway and down-regulating the level of inflammatory factors in mice.
