Isolation,identification and characteristics of poultry Clostridium perfringens in the Yangzhou region of Jiangsu Province
10.16303/j.cnki.1005-4545.2025.10.15
- VernacularTitle:江苏省扬州地区禽源产气荚膜梭菌的分离鉴定及其特性分析
- Author:
Haiping XIE
1
;
Zewei LI
1
;
Yifei CHEN
1
;
Huoying SHI
1
Author Information
1. 扬州大学兽医学院江苏省动物重要疫病与人兽共患病防控协同中心,江苏扬州 225009
- Publication Type:Journal Article
- Keywords:
Clostridium perfringens;
avian;
toxin typing;
antimicrobial susceptibility testing;
multi-locus sequence typing
- From:
Chinese Journal of Veterinary Science
2025;45(10):2206-2212,2221
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to understand the epidemiological and biological character-istics of Clostridium perfringens in avian origin in Yangzhou.A total of 200 intestinal samples(90 from chickens and 110 from ducks)collected from different markets were isolated and identified by isolation and culture,morphological observation,staining microscopy,biochemical tests and other methods,antimicrobial susceptibility test by K-B paper method,and toxin type of isolated bacteria was detected by PCR.Five isolates were screened according to the characteristics of host origin,se-rotype and drug resistance,and multi-locus sequence typing analysis was carried out,and a phylo-genetic tree based on α toxin gene sequences was constructed for isolates from different sources.After morphological and molecular biological identification,a total of 61 strains of Clostridium perfringens were isolated,and the isolation rate was 30.5％,all of which were type A.The antimi-crobial susceptibility results showed that the isolate had the highest resistance rate to amikacin,followed by tetracycline and cotrimoxazole,and was more sensitive to ceftriaxone,penicillin,amoxicillin and imipenem.Multi-drug resistance is very serious,with six or more strains resistant to antibiotics emerging in every market.A total of five different ST types(STs)were found by multi-site sequence analysis,namely ST833,ST834,ST363,ST835,and ST837,and all of them were new ST types except ST363.The information from ST 363 is consistent with clinical isolate of Clostridium perfringens,suggesting that more attention needs to be paid to zoonotic diseases caused by Clostridium perfringens.The α toxin genes of the five isolates were located in four dif-ferent branches in the phylogenetic tree,indicating their genetic diversity,while the isolates from different provenances of the same source were located in the same branch,indicating that they had a closer evolutionary relationship.The Clostridium perfringens from poultry in Yangzhou are mainly type A,and with severe drug resistance and multi-drug resistance present.This research provides scientific evidence for the epidemiology and control of Clostridium perfringens.
