Isolation,identification and pathogenicity analysis of two avain infectious bronchi-tis virus strain
10.16303/j.cnki.1005-4545.2025.10.06
- VernacularTitle:2株鸡传染性支气管炎病毒株的分离鉴定及致病性分析
- Author:
Hanwen ZHANG
1
;
Yaru GAO
1
;
Yang WANG
1
;
Yapeng SONG
1
;
Wenming GAO
1
;
Lin LIU
1
;
Xiaoyang CAO
1
;
Jingrui LIU
1
;
Xinsheng LI
1
Author Information
1. 河南农业大学动物医学院,河南郑州 450002
- Publication Type:Journal Article
- Keywords:
chicken infectious bronchitis virus;
gene sequence analysis;
genetic variation;
pathogenic-ity
- From:
Chinese Journal of Veterinary Science
2025;45(10):2133-2141,2155
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate the isolate and identify of infectious bronchitis virus(IBV)in chickens,and study its genetic variation and pathogenicity.In 2023,two strains named CK/CH/HN/SQ202301 and CK/CH/HN/SQ202302 were obtained from suspected infectious bronchitis(IB)infected materials collected in a region of Henan Province,China.Further analysis showed that the two isolates belong to the G Ⅰ-13 and GⅥ genotypes,respectively.The cleavage sites of S protein were all RRSRR.The prediction of glycosylation sites showed that the two isolates had 18 and 12 N-glycosylation sites respectively,but no O-glycosylation site.Recombinant analysis shows that C2023-1 was a recombinant strain.Pathogenicity was assessed by infecting 1-day-old SPF chicks with the two isolates,and the results showed that C2023-1 strain infection could cause clini-cal symptoms such as depression and head shaking,as well as death in chicks,with a mortality rate of 37.5％.There were no clinical symptoms or deaths after infection with C2023-2 strain.Viral load test results showed that both isolates continued to detoxify until the 10th day,and had strong rep-lication capacity in the kidney,trachea and bursa of Fabricius.The results indicate significant differences in the genetic characteristics and pathogenicity of the two isolates due to their different genotypes.This study not only provides new epidemiological data on IB,which contributes to a bet-ter understanding of IBV's epidemiological features and control challenges,but also adds valuable bioinformatics resources for IBV by analyzing its variation mechanisms and biological information.
