Isolation,identification and virulence evaluation of clinical strains of Actinobacil-lus pleuropneumoniae serotype 15 in pigs
10.16303/j.cnki.1005-4545.2025.10.09
- VernacularTitle:猪胸膜肺炎放线杆菌血清15型临床菌株的分离鉴定及其毒力评价
- Author:
Lu PENG
1
;
Jiao ZHANG
;
Weiyao HAN
;
Qiuhong ZHANG
;
Zhen LUO
;
Bo HONG
;
Zhichang LIU
;
Rui ZHOU
;
Yunfeng SONG
;
Lu LI
Author Information
1. 华中农业大学动物医学院农业微生物资源发掘与利用全国重点实验室/生猪健康养殖省部共建协同创新中心,湖北武汉 430070
- Publication Type:Journal Article
- Keywords:
Actinobacillus pleuropneumoniae;
clinical isolates;
serotype 15;
biological characteris-tics;
virulence in piglets
- From:
Chinese Journal of Veterinary Science
2025;45(10):2156-2162,2230
- CountryChina
- Language:Chinese
-
Abstract:
In recent years,the prevalence of porcine infectious pleuropneumonia caused by Actinoba-cillus pleuropneumoniae(APP)has significantly increased in large-scale pig farms in China.The i-solation,identification,and biological characteristic testing of newly prevalent strains have impor-tant clinical significance.This study isolated four suspected APP strains(named XB2T-56,JMTLP-443,YD-THB-755,SYZJ-291)from lung samples of pig farms suspected of outbreaks of porcine infectious pleuropneumonia in Hubei Province.They were identified by PCR amplification,and multiplex PCR serological typing,the biological properties were tested by detecting the growth a-bility,hemolytic activity and biofilm forming ability.The drug resistance of the strains was ana-lyzed by drug sensitivity testing,and the virulence of one strain(XB2T-56)in mice and piglets was evaluated.The PCR identification result showed that all four clinical isolates were APP serotype 15 strains.These four strains grew slower than serotype 1 standard strains,had weaker hemolytic activity,but could form stronger biofilms.These strains are all less sensitive to cephalosporin,tylo-sin,sulfamethoxazole,and doxycycline,but sensitive to cefotaxime and enrofloxacin.The mice and piglet infection experiments showed that the isolated strain XB2T-56 has strong virulence.Artifi-cial intranasal infection causes acute death in piglets,with typical symptoms of pleuropneumonia in the lungs,and serotype 15 strains can be isolated from the lesion site.This study indicates that the APP serotype 15 strain has strong virulence,providing new date for the clinical epidemiological characteristics of APP.
