Genome sequence and bioinformatics analysis of an ovine-derived Salmonella enterica strain
10.16303/j.cnki.1005-4545.2025.10.13
- VernacularTitle:1株羊源沙门菌全基因组序列测定与生物信息学分析
- Author:
Yao WANG
1
;
Longbin KANG
;
Renjie WU
;
Qiuyong CHEN
;
Longbai WANG
;
Dingping BAI
;
Lunjiang ZHOU
;
Yongliang CHE
Author Information
1. 福建农林大学动物科学学院福建省兽医中药与动物保健重点实验室,福建 福州 350002;福建省农业科学院畜牧兽医研究所福建省畜禽疫病防治工程技术研究中心,福建 福州 350013
- Publication Type:Journal Article
- Keywords:
sheep;
Salmonella;
whole genome sequencing;
integrated bonding element;
plasmid;
bioinformatics
- From:
Chinese Journal of Veterinary Science
2025;45(10):2187-2195
- CountryChina
- Language:Chinese
-
Abstract:
To elucidate the genomic characteristics of Salmonella strains derived from sheep,this study employed various methods,including bacterial isolation and identification,biochemical identi-fication,pathogenicity test,whole-genome sequencing,and BLAST comparison,along with the screening of integrative conjugative elements(ICE)using ICEfinder and EasyFig for comparative analysis,as well as plasmid comparisons utilizing PlasmidBrig.The results revealed the isolation of a Gram-negative,non-spore-forming bacillus from nasal swabs of diseased sheep,which formed gray-white,smooth-surfaced,and neatly edged circular colonies on TSA sheep blood agar.On XLT-4 agar medium,it produced smooth-surfaced,white,circular colonies.The bacterium was identified as Salmonella enterica through 16S rRNA sequencing and biochemical identification.This bacteri-um induces hemorrhaging in the intestines of guinea pigs,resulting in their demise within a 48-hour period.The pathogen exhibits high virulence.Whole-genome alignment demonstrated a high degree of homology with Salmonella enterica subsp.diarizonae serotype 61:k:1,5,(7).ICE screen-ing and comparative analysis indicated the presence of a novel ICE in this strain,characterized by a core structural framework that includes an integrative shear module,a mobilizable processing mod-ule,a conjugative pair formation module,and a regulatory module.Notably,ICE from different spe-cies containing the same integrase exhibited identical inverted repeat sequences and insertion sites at tRNAPhe.Plasmid homology comparisons revealed that plasmid sequences from different strains of Salmonella enterica subsp.diarizonae serotype 61:k:1,5,(7)also showed high homology;however,the homology with plasmid sequences from other Salmonella and Escherichia coli strains was only 50％.These findings indicate that the isolated strain is Salmonella enterica subsp.diarizonae serotype 61:k:1,5,(7)and contains a novel ICE as well as a plasmid.This study fur-ther enriches the molecular epidemiology of Salmonella and provides a theoretical basis for the prevention and control of infections caused by this pathogen.
