Mechanism of Huazhuo Xingxue Decoction on the Treatment of Ischemic Stroke Based on Network Pharmacology
10.11842/wst.20240708005
- VernacularTitle:基于网络药理学探讨化浊行血汤对缺血性脑卒中的治疗机制
- Author:
Meng CHEN
1
;
Yuejin DU
;
Chunli GUO
;
Nana WANG
;
Fei HOU
;
Yuchen ZHANG
;
Zipeng DIAO
;
Juaner ZHENG
;
Qiang FU
Author Information
1. 中国标准化研究院 北京 100191
- Publication Type:Journal Article
- Keywords:
Huazhuo Xingxue Decoction;
Ischemic stroke;
Network pharmacology;
Molecular docking;
Oxygen glucose deprivation/reoxygenation cell model;
TNF-α
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2025;27(5):1461-1470
- CountryChina
- Language:Chinese
-
Abstract:
Objective The mechanism of Huazhuo xingxue decoction(HZXXD)in the treatment of ischemic stroke was explored through network pharmacology,molecular docking and cell validation.Methods TCMSP,TCMID,BATMAN-TCM database and literature search were used to get the chemical components and related target proteins of Huazhuo Xingxue Decoction,and the targets of dementia,stroke and amnesia were obtained from Genecards database and OMIM database.The traditional Chinese medicine-active components-target-network and protein interaction map were constructed by using Cytoscape,and the target was enriched by KEGG pathway by David database.Western blot was used to investigate the effect of HZXXD on inflammation-related core targets expression using oxygen and glucose deprivation/reoxygenation cell model.Finally,Autodock was used for molecular docking of key active ingredients and important targets to evaluate their binding activity.Results 76 active molecules and 33 common targets of herb-disease were screened out.KEGG bioaccumulation results involve multiple inflammatory signal pathways such as TNF,chemical carcinogenesis-reactive oxygen species and HIF-1.TNF-α was found to be the core target of HZXXD by oxygen glucose deprivation/reoxygenation cell experiments.Five compounds with the strongest binding ability to TNF-α,kaempferol,apigenin,aloe-emodin,baicalein and stigasterol,were screened by traditional Chinese medicine-active ingredient-target network map and molecular docking.Conclusion Huazhuo Xingxue Decoction may down regulate the expression of core target TNF-α,kaempferol,apigenin,aloe emodin,baicalein and stigasterol may be the main active substances for TNF-α binding.
