To Explore the Mechanism of Kanggan Mixture Intervene in Rats with Acute Lung Injury Based on Target Network
- VernacularTitle:基于靶点网络的抗感合剂干预急性肺损伤大鼠作用机制研究
- Author:
He HUANG
1
;
Zhongying GUAN
1
;
Decheng WANG
1
;
Jinghe ZHU
1
;
Boyu JING
1
;
Yu ZHANG
1
;
Xiaohu LIU
1
;
Tiefa GUAN
1
;
Yinglan FAN
1
;
Yu GAN
1
;
Lei ZHAO
1
Author Information
- Publication Type:Journal Article
- Keywords: Kanggan Mixture(KGM); Network pharmacology; micro-CT; Acute lung injury; Molecular mechanism
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2025;27(5):1447-1460
- CountryChina
- Language:Chinese
- Abstract: Objective To elucidate the molecular mechanisms underlying the effects of Kanggan Mixture(KGM)on key targets in rats with acute lung injury,network pharmacology and in vivo micro-CT experiments were employed.Methods Network pharmacology was utilized to forecast the target genes and principal pathways involved in the intervention of KGM in acute lung injury(ALI).Lipopolysaccharide(LPS)-induced ALI rat models were utilized,and micro-computed tomography(micro-CT)was employed to evaluate the extent of lung injury in vivo.Experiments were conducted to verify the intervention mechanism of KGM on ALI rats.Results The findings revealed that 190 chemical constituents were identified from KGM,and 579 potential targets and 204 pathways associated with KGM's impact on ALI were predicted.The principal components of KGM,such as quercetin,luteolin,kaempferol,betulin,and lupenone,exhibit anti-viral,anti-inflammatory,and immunomodulatory properties by targeting TP53,AKT1,SRC,EP300,and STAT3,and modulating the FoxO signaling pathway,TNF signaling pathway,PI3K-Akt signaling pathway,and MAPK signaling pathway,demonstrating an influence on acute lung injury.Micro-CT results suggest that KGM can improve lung texture enhancement and lung injury in ALI rats,with an increase in end-expiratory lung volume(inspiratory phase-expiratory phase).The HE and W/D ratio results indicate that KGM can improve lung tissue injury and reduce the lung tissue wet/dry weight ratio(P<0.01).Blood cell analysis results show that the anti-inflammatory agent can decrease the WBC(white blood cell count)and N%(neutrophil percentage)in ALI rats'blood(P<0.01),and increase lymphocytes(P<0.05).Real-time quantitative PCR,WES,and immunohistochemistry results suggest that KGM can decrease the mRNA expression,protein distribution,and protein expression levels of TP53,AKT1,SRC,EP300,and STAT3 in lung tissue of ALI rats(P<0.05).Conclusion KGM has a certain intervention effect on acute lung injury,mainly achieved through the core targets STAT3,EP300,SRC,AKT1,and TP53.
