Efficacy and Mechanism of Tanreqing Injection in the Treatment of Acute Exacerbation of Chronic Obstructive Pulmonary Disease Based on Network Pharmacology
10.11842/wst.20240618008
- VernacularTitle:痰热清注射液治疗慢性阻塞性肺疾病急性加重的疗效及网络药理学机制
- Author:
Yunxia LIN
1
;
Zhu ZHU
1
Author Information
1. 南京脑科医院胸科院区 南京 210029
- Publication Type:Journal Article
- Keywords:
Acute exacerbation of chronic obstructive pulmonary disease;
Tanreqing injection;
Clinical efficacy;
Network pharmacology;
Cell experiment
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2025;27(5):1434-1446
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the clinical efficacy and mechanism of Tanreqing injection on patients with acute exacerbation of chronic obstructive pulmonary disease(AECOPD)based on network pharmacology and cell experiments.Methods The 80 patients with AECOPD were divided into a control group and a combination group of 40 cases according to the random number method.Both groups were given conventional Western medicine treatment,and the combined group was treated with Tanreqing injection.The clinical efficacy of TCM,inflammatory factors and safety were compared between the two groups of patients.Databases such as TCMSP and software such as Cytoscape 3.7.0 were used to screen out the mechanism of Tanreqing injection in the treatment of AECOPD,and human bronchial epithelial cells 16HBE were used for experimental verification.Results The clinical effective of TCM in the combined group was higher than that in the control group(P<0.05).WBC,hs-CRP,IL-6 and SAA in the two groups were lower than before treatment(P<0.05),and those of the combined group were lower than control group(P<0.05).There was no statistically difference in adverse reactions between the two groups(P>0.05).Key targets such as MAPK1,IL-6,AKT1,and key pathways such as IL-17 signaling pathway and PI3K-Akt signaling pathway were obtained by network pharmacology methods.The level of MAPK1,IL-6,p-AKT/AKT and PI3K in the model group were significantly lower than the blank group(P<0.01);Compared with the model group,the expression of MAPK1,IL-6,pAKT/AKT and PI3K protein in the Tanreqing extract group significantly and dose-dependently reduced(P<0.05).Conclusion Tanreqing injection can reduce the inflammation level and improve the clinical efficacy in AECOPD patients.Its mechanism may be related to the inhibition of protein expression including IL-6,PI3K and p-AKT/AKT,and the regulation of the PI3K-Akt signaling pathway.
