Comprehensive Quality Evaluation of Camptothecae Fructus Medicinal Materials Based on HPLC-QAMS Multi-Components Quantification Combined with OPLS-DA and Weighted TOPSIS Model
- VernacularTitle:基于HPLC-QAMS多指标成分联合OPLS-DA及加权TOPSIS模型的喜树果药材质量评价
- Author:
Siyang LI
1
;
Gengdong LI
;
Yuhui YAN
Author Information
- Publication Type:Journal Article
- Keywords: Camptothecae Fructus; HPLC; Chemometrics; PCA; OPLS-DA; Weighted TOPSIS; Quality evaluation
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2025;27(5):1376-1385
- CountryChina
- Language:Chinese
- Abstract: Objective To screen the differential markers affecting their quality,and sort their quality,a method for simultaneous detection of 9 components in Camptothecae Fructus from different producing areas was established.Methods Reflux extraction was performed on 18 batches of Camptothecae Fructus from 7 provinces,and the extracts were detected by HPLC.A orthogonal partial least squares discriminant analysis and weighted TOPSIS method were used to establish a quality evaluation model for Camptothecae Fructus,and the quality differences were comprehensively evaluated.Results When the 9 components were in the 0.51-12.75,0.23-5.75,3.21-80.25,4.45-111.25,1.88-47.00,0.41-10.25,2.05-51.25,0.34-8.50 and 7.95-198.75 μg·mL-1,their linear relationships were good(r>0.999).The average recovery were 96.95%-100.06%with the RSDs<2.0%.18 batches of samples were clustered into 3 categories.Vincosamide,10-hydroxycamptothecin,camptothecin 10-methoxycamptothecin and pumiloside may be the main potential markers affecting the quality of Camptothecae Fructus.The analysis results of the weighted TOPSIS method revealed that the closeness(Jb)for evaluating the quality of 18 batches of Camptothecae Fructus ranged from 0.1090 to 0.7385,with S14 achieving the highest value of 0.7385.Conclusion A method for simultaneous determination of 9 components in Camptothecae Fructus was established,which is simple and accurate.OPLS-DA and weighted TOPSIS method can be used to evaluate the quality difference of Camptothecae Fructus.
