The alleviation effect of Cornuside on Intestinal Injury in Rats with Septic Shock by Inhibiting TREM1-Mediated M1 Polarization of Macrophages
10.11842/wst.20240605002
- VernacularTitle:山茱萸新苷通过抑制TREM1介导的巨噬细胞M1型极化减轻感染性休克大鼠肠道损伤
- Author:
Aibin CHENG
1
;
Jinyu LI
1
;
Xuan BU
1
;
Jianjun WANG
1
;
Kai FENG
1
;
Jing BAI
1
;
Jian LIU
1
Author Information
1. 华北理工大学附属医院 唐山 063000
- Publication Type:Journal Article
- Keywords:
Cornuside;
Septic shock;
Intestinal injury;
Polarization of macrophages;
Triggering receptor expressed on myeloid cells-1
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2025;27(5):1351-1359
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of cornuside on intestinal injury in rats with septic shock,and clarify its possible mechanism.Methods SD rats were randomly divided into normal control group,model group,low-,medium-,and high-dose comecarpine glycosides groups,and TREM1 inhibitor(LR12)group.HE staining was used to observe the pathological injury of small intestinal mucosa.The levels of D-lactic acid(D-LA)and diamine oxidase(DAO)in serum and secretory immunoglobulin(sIg A)in small intestine were detected by ELISA.Intestinal mucosal permeability was detected by fluorescein isothiocyanate-dextran(FITC-D)tracer method.ELISA was used to detect the levels of interferon(IFN)-γ,tumor necrosis factor(TNF)-α,interleukin(IL)-1β,IL-10 and arginase(Arg)-1 in serum.The polarization of macrophages in small intestinal tissue was detected by flow cytometry.Western blot was used to detect the protein expression levels of triggering receptor expressed on myeloid cells 1(TREM1),CD86 and CD206 in small intestine.Results Compared with the normal control group,the model group had serious pathological injury of the small intestinal mucosa,and the serum levels of D-LA,DAO,FITC-D,IFN-γ,TNF-α,and IL-1β significantly increased(P<0.05),while the levels of sIg A,IL-10,and Arg-1 significantly decreased(P<0.05).The M1/M2 ratio of macrophages and the expression levels of TREM1 and CD86 proteins in the small intestine tissue significantly increased(P<0.05),while the expression level of CD206 protein significantly decreased(P<0.05).Compared with the model group,the small intestinal mucosal injury of the rats in each dose cornuside group and LR12 group significantly improved,and the serum levels of D-LA,DAO,FITC-D,IFN-γ,TNF-α,and IL-1β significantly decreased(P<0.05),while the levels of sIg A,IL-10,and Arg-1 significantly increased(P<0.05).The M1/M2 ratio of macrophages and the expression levels of TREM1 and CD86 proteins in the small intestine tissue significantly decreased(P<0.05),while the expression level of CD206 protein significantly increased(P<0.05).Conclusion Cornuside can reduce intestinal injury in rats with septic shock,and the mechanism may be related to inhibiting TREM1-mediated M1 polarization of macrophages.
