The variation characteristics of Siglec-9 in pulmonary tuberculosis
10.13431/j.cnki.immunol.j.20240072
- VernacularTitle:负性免疫分子Siglec-9在肺结核中的变化特点
- Author:
Yueyue HE
1
;
Yuejie ZHU
;
Zhengwei YIN
;
Juan SHI
;
Kaiyu SHANG
;
Tingting TIAN
;
Huidong SHI
;
Jianbing DING
;
Fengbo ZHANG
Author Information
1. 830011 乌鲁木齐,新疆医科大学中亚高发疾病发病机制、防治国家重点实验室和基础医学院
- Publication Type:Journal Article
- Keywords:
Siglec-9;
PTB;
CD4+T cell;
Bioinformatics;
Negative immunoregulation
- From:
Immunological Journal
2024;40(6):526-532
- CountryChina
- Language:Chinese
-
Abstract:
This study was performed to investigate the variation characteristics and functions of Siglec-9 immune checkpoint in pulmonary tuberculosis.R language was used for bioinformatics analysis of GSE83456 chip data.Total of 48 patients with confirmed pulmonary tuberculosis(PTB)and 46 healthy controls were included.Real-time fluorescence quantitative PCR(qRT-PCR)was used to detect the mRNA expression of Siglec-9 in peripheral blood;flow cytometry was used to detect the proportion of Siglec-9+CD4+T cells.The levels of TNF-α,IL-6,IFN-γ and IL-4 were detected by Cytometric Bead Array(CBA).Furthermore,the correlation of the proportion of Siglec-9+CD4+T cells with TNF-α and other cytokines were analyzed.Bioinformatics analysis showed that Siglec-9 was significantly increased in patients with PTB(P＜0.001),and was related to TNF-α/NF-κB,IL-6/JAK/STATA3,PI3K/AKT/mTOR signal pathways.Experimental data showed that the proportion of Siglec-9+CD4+T cells was significantly increased in patients with PTB(P＜0.001)and negatively correlated with the levels of TNF-α and other cytokines.In conclusion,the higher levels of Siglec-9 mRNA and Siglec-9+CD4+T cells in PTB may inhibit the function of CD4+T cells and participate in the occurrence and development of PTB.
