Effect of sRNA OxyS on pathogenicity of Salmonella typhimurium
10.16303/j.cnki.1005-4545.2025.05.17
- VernacularTitle:非编码小RNA OxyS对鼠伤寒沙门菌生物学特性及致病性的影响
- Author:
Xiaochen LIU
1
;
Xiaoyu ZHANG
;
Siping ZHU
;
Hong LI
;
Chihuan LI
;
Yulai DONG
;
Zhiqiang ZHANG
;
Qiumei SHI
Author Information
1. 河北科技师范学院河北省预防兽医学重点实验室,河北秦皇岛 066004
- Publication Type:Journal Article
- Keywords:
Salmonella typhimurium;
λ-Red homologous recombination;
gene deletion;
OxyS
- From:
Chinese Journal of Veterinary Science
2025;45(5):1009-1016
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the role of sRNA OxyS in the pathogenicity of Salmonella typhi-murium infection,the OxyS gene deletion strain ATCC25241 △OxyS and the back-complemented strain ATCC25241 △OxyS/OxyS of Salmonella typhimurium ATCC25241 were constructed by using λRed homologous recombination technique.We investigated the effect of OxyS deletion on the biological characteristics and pathogenicity of Salmonella typhimurium ATCC25241.The re-sults showed that the deletion of OxyS did not affect the growth rate,the ability of biofilm forma-tion,and the ability of adhesion,invasion and intracellular survival of Salmonella typhimurium,but significantly reduced the motility of Salmonella typhimurium as well as its ability to survive in alkaline and oxidative environments.The results of mouse infection test showed that OxyS dele-tion caused a significant decrease in the virulence of Salmonella typhimurium in mice,and toxicity is reduced obviously.The qPCR results also showed that OxyS deletion could lead to changes in the transcript levels of a number of virulence-related genes of Salmonella typhimurium such as pipB,orf245,csgA,invH,tatA,sipA,sipB,and so on.The above results indicate that OxyS gene affects the biological characteristics and pathogenicity of Salmonella typhimurium and is an important virulence regulator of Salmonella typhimurium.
