Polypeptide SJMHE1 plays a therapeutic role in asthmatic mice by regulating miR-21 and miR-17 expression
10.13431/j.cnki.immunol.j.20240066
- VernacularTitle:多肽SJMHE1通过调节miR-21和miR-17的表达对哮喘小鼠发挥治疗作用
- Author:
Yu ZHENG
1
;
Chao DING
1
;
Long CHEN
1
;
Guoxin XU
1
Author Information
1. 215600 张家港,苏州大学附属张家港医院检验科
- Publication Type:Journal Article
- Keywords:
SJMHE1;
Asthma;
microRNA;
T helper cells;
p-STAT3;
PTEN
- From:
Immunological Journal
2024;40(6):489-495
- CountryChina
- Language:Chinese
-
Abstract:
This study was designed to explore the therapeutic effect of the Schistosoma japonicum-derived peptide SJMHE1 in asthmatic mice and the roles of microRNA-21(miR-21)and miR-17 in this process.Mice were randomly divided into control group,asthma group,asthma+PBS group,and asthma+SJMHE1 group.Asthma model was induced with the ovalbumin(OVA)in mice.Airway inflammation was assessed by histopathologic sections of mouse lungs;bronchoalveolar lavage fluid(BALF)was sorted and counted,and the expression level of OVA-specific IgE in mouse serum was measured by ELISA;the distribution of T helper(Th)cells was analyzed by flow cytometry.qRT-PCR was used to detect the expression of Th-associated transcription factors and cytokines,the levels of miR-21 and miR-17 in the lungs of mice;Western blot was used to detected the protein expression of phosphorylated signal transducer and activator of transcription 3(p-STAT3)and phosphatase and tensin homolog(PTEN).Compared with the control group,mice in the asthma group demonstrated increases in airway inflammation,eosinophil and neutrophil counts in the BALF,serum IgE expression levels(P＜0.001),proportions of Th2 and Th17 cells,expressions of related transcripts and cytokines(P＜0.05),expression of miR-21 and miR-17(P＜0.001),and expression of p-STAT3,while decreases in proportions of Th1 and Treg cells,expression of related transcripts and cytokines,and expression of PTEN.All indicators mentioned above was reversed in mice of the asthma/SJMHE1 group,as compared with the asthma/PBS group.In conclusion,the peptide SJMHE1 regulates Th1/Th2/Th17/Treg cell balance via miR-21 and miR-17,thus plays a therapeutic role in asthmatic mice.
