Analysis of immunogenicity of African swine fever virus p37 recombinant protein in mice
10.16303/j.cnki.1005-4545.2025.05.01
- VernacularTitle:非洲猪瘟病毒p37重组蛋白对小鼠免疫原性分析
- Author:
Ying HUANG
1
;
Wenzhu ZHAI
;
Chunhao TAO
;
Yuheng HE
;
Zhen WANG
;
Yuanyuan CHU
;
Zhongbao PANG
;
Hongfei ZHU
;
Hong JIA
Author Information
1. 中国农业科学院北京畜牧兽医研究所,北京 100193
- Publication Type:Journal Article
- Keywords:
African swine fever;
p37 protein;
immunogenicity;
humoral immunity;
cellular immunity
- From:
Chinese Journal of Veterinary Science
2025;45(5):889-895
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study is to explore the immunogenicity of African swine fever virus p37 recombinant protein in mice.C57BL/6J mice were immunized subcutaneously in the abdomen using p37 recombinant protein as antigen.The second immunization was performed 21 d after the first immunization.Serum-specific antibody levels were detected by ELISA;serum cytokine levels were detected using a multifactor assay technique;mice splenic lymphocytes were isolated 7 d after sec-ondary immunization,and the number of splenic lymphocytes secreting IFN-γ after recombinant protein stimulation was detected by ELISpot;and the ratio of CD4+T cells to CD8+T cells was detected by flow cytometry.The results of indirect ELISA showed that p37 recombinant protein could stimulate mice to produce high levels of specific antibodies;ELISpot showed that p37 recom-binant protein could significantly stimulate splenic lymphocytes to produce IFN-γ(P＜0.001)and activate cellular immune responses;the results of flow cytometry showed that it could signifi-cantly stimulate the differentiation of T-lymphocytes to CD4+T-lymphocytes(P＜0.001).In ad-dition,serum levels of IL-2,IL-4,IFN-γ,and TNF-α immune-related cytokines were significantly higher after the second immunization.Immunization of mice with p37 recombinant protein induced strong humoral and cellular immune responses with good immunogenicity,providing reference for the subsequent epitope identification and functional study of p37 protein and the antigen screening of ASF mRNA vaccine.
