Effects of salidroside regulates UCHL1 expression and inhibits the apoptosis of keloid fibroblasts
10.13699/j.cnki.1001-6821.2024.22.011
- VernacularTitle:红景天苷调控UCHL1表达抑制瘢痕疙瘩成纤维细胞凋亡的作用
- Author:
Yi-yang ZHANG
1
;
Shun XU
;
Yan LU
Author Information
1. 上海中医药大学研究生院,上海 201203
- Publication Type:Journal Article
- Keywords:
salidroside;
keloid;
fibroblast;
ubiquitin C-terminal hydrolase l1;
ferroptosis
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(22):3270-3274
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of salidroside on abnormal proliferation and extracellular matrix deposition of keloid(KD)fibroblasts.Methods The primary KD fibroblasts were randomly divided into model group(normal culture),experimental-L group(50 μmol·L-1 salidroside),experimental-H group(100 μmol·L-1 salidroside),Vector group(100 μmol·L-1 salidroside+Vector),ubiquitin C-terminal hydrolase L1(UCHL1)group(100 μmol·L-1 salidroside+overexpression UCHL1);The mRNA expression level of UCHL1 was detected by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR);flow cytometry was used to detect apoptosis;Western blot assay was used to detect the expression levels of Collagen Ⅰ,glutathione peroxidase 4(GPX4)and α-smooth muscle actin(α-SMA);the reactive oxygen species(ROS)levels was detected by DCFH-DA method.Results The relative expression levels of UCHL1 mRNA in model group,experimental-L,experimental-H,vector group and UCHL1 group were 1.01±0.11,0.71±0.07,0.49±0.05,0.50±0.05 and 2.36±0.13,respectively;the apoptosis rates were(4.59±0.56)％,(12.11±0.80)％,(22.16±3.21)％,(23.54±2.08)％,(9.04±0.72)％,respectively;Collagen Ⅰ protein expression levels were 0.97±0.06,0.82±0.07,0.53±0.04,0.54±0.07 and 0.91±0.11,respectively;α-SMA protein expression levels were 1.11±0.07,0.79±0.06,0.61±0.08,0.60±0.04,0.96±0.11,respectively;GPX4 protein expression levels were 0.93±0.06,0.66±0.04,0.41±0.03,0.43±0.03,0.78±0.04,respectively;ROS levels were(100.00±5.03)％,(127.18±10.15)％,(146.09±10.15)％,(144.75±10.95)％,(106.57±11.28)％,respectively.Compared with the model group,the above indexes in the experimental-L,-H groups were statistically significant(all P＜0.05).Compared with the Vector group,the above indexes in the UCHL1 group were statistically significant(all P＜0.05).Conclusion Salidroside can induce iron death,promote fibroblast apoptosis,inhibit excessive extracellular matrix deposition,and slow down KD progression,which may be related to the inhibition of UCHL1 expression.
