Screening and efficacy evaluation of cross-immunological protective antigen Pm-CQ2-5175 of bovine Pasteurella multocida
10.16303/j.cnki.1005-4545.2025.05.11
- VernacularTitle:牛多杀性巴氏杆菌交叉免疫保护性抗原蛋白PmCQ2-5175的筛选及效果评价
- Author:
Pan XIONG
1
;
Yanlan HUANG
1
;
Siyu LIU
1
;
Liu YANG
1
;
Guangfu ZHAO
1
;
Nengzhang LI
1
;
Fang HE
1
;
Yuanyi PENG
1
Author Information
1. 西南大学 动物医学院,重庆 北碚 400715
- Publication Type:Journal Article
- Keywords:
Pasteurella multocida;
immune protection;
recombinant protein;
mice
- From:
Chinese Journal of Veterinary Science
2025;45(5):963-970
- CountryChina
- Language:Chinese
-
Abstract:
Pasteurella multocida(Pm)type A is an important pathogen responsible for respiratory diseases,such as bovine pneumonia,which seriously restricts the development of cattle industry in China.Currently,the prevention of Pm infection-related diseases primarily relies on vaccination in production.However,the diverse Pm serotypes result in inadequate cross-immunological protection from vaccines.Therefore,it is of great significance to develop vaccines with cross-protection for the prevention and control of Pm infectious diseases.The previous studies conducted by our team have demonstrated that PmCQ2△cra exhibits a strong immune protective effect against Pm type A(PmA),Pm type B(PmB),and Pm type F(PmF).Transcriptomic sequencing results suggest that the cross-immunoprotective effect of PmCQ2△cra may be attributed to high expression levels of bacterial surface protective antigens.Consequently,four putative immune protective antigens,namely PmCQ2-5175,PmCQ2-6290,PmCQ2-0275 and PmCQ2-2640,were screened through bioin-formatics analysis in this study.Subunit vaccines formulated with these potential antigenic proteins exhibited protective efficacy of 62.5％,25％,12.5％and 10％against PmA-infected mice,respectively.Importantly,PmCQ2-5175,one of the most protective single-component antigen vac-cines,demonstrating a 75％cross-protection against PmB infection in mice.Furthermore,the pro-tective efficacy of the PmCQ2-5175 protein screened in this study was superior to that of the previ-ously reported Pm antigen protein plpE.Moreover,the fusion expression protein PmCQ2-5175-PLPE exhibited better protective effects against PmA compared to a single protein.The findings of this study will establish a theoretical foundation for the advancement of Pm subunit vaccines with broad-spectrum immune protection.
