Effects of Hedysari Radix Praeparata Cum Melle and vinegar processed Curcumae Rhizomaon short-chain fatty acid metabolism in colitis-related colon cancer mice
10.13699/j.cnki.1001-6821.2024.22.019
- VernacularTitle:炙红芪-醋莪术对结肠炎相关结肠癌小鼠短链脂肪酸代谢的影响
- Author:
Fei-yun GAO
1
;
Ting LIU
1
;
Zhuan-hong ZHANG
1
;
Yan-jun WANG
1
;
Yu-gui ZHANG
1
;
Yu-jing SUN
1
;
Yue-feng LI
1
Author Information
1. 甘肃中医药大学药学院,甘肃兰州 730000;甘肃中医药大学科研实验中心,甘肃兰州 730000
- Publication Type:Journal Article
- Keywords:
Hedysari Radix Praeparata Cum Melle and vinegar processed Curcumae Rhizoma;
short-chain fatty acid;
colitis-associated colon cancer;
gas chromatography-mass spectrometry
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(22):3312-3317
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Hedysari Radix Praeparata Cum Melle and vinegar processed Curcumae Rhizoma(HRCR)on short-chain fatty acids(SCFAs)in colitis associated colon cancer(CAC)mice.Methods Forty C57BL/6 mice were randomly divided into control group,model group,sulfasazine group(SASP)and HRCR group,with 10 mice per group.Control group was given the same amount of drinking water,and the other group was given azoxymethane combined with dextran sulfate sodium to establish an CAC model.The SASP and HRCR groups were administrated with 0.455 g·kg-1 sulfasalazine and 6.825 g·kg-1 HRCR from the beginning of the experiment.The content of SCFAs in colon contents of mice was detected by gas chromatography-mass spectrometry.Results The contents of acetic acid,propionic acid,isobutyric acid,butyric acid,valeric acid,and hexanoic acid in the colon contents of control group mice were(2.80±0.54),(0.47±0.26),(0.02±5.90 x 10-3),(0.82±0.24),(9.85±2.24)×10-3,(7.21±4.21)×10-3 mg·g-1 respectively.Compared with the control group,the contents of acetic acid,propionic acid,isobutyric acid,butyric acid and valeric acid in the colon contents of mice in the model group were significantly reduced[(0.71±0.36),(0.24±0.08),(6.08±2.54)× 10-3,(0.13±0.03),(3.64±2.14)×10-3 and(1.99±0.81)×10-3 mg·g-1,all P＜0.01].Compared with the model group,the contents of acetic acid,propionic acid,butyric acid,valeric acid,and hexanoic acid in the colon contents of mice treated with HRCR were significantly increased after intervention[(1.58±0.25),(0.39±0.04),(0.34±0.08),(5.51±1.76)×10-3 and(6.67±1.73)×10-3 mg·g-1,P＜0.05,P＜0.01].Conclusion The metabolism of SCFAs in CAC mice can be regulated by decocted astragalus-vinegar Curcuma.
