Establishment of a rapid fluorescence immunochromatographic assay for avian influenza virus subtype H5N6
10.3969/j.issn.1002-2694.2025.00.035
- VernacularTitle:禽流感病毒H5N6亚型快速荧光免疫层析检测方法的建立
- Author:
Hui LI
1
;
Li LIU
;
Yi-sheng ZHOU
;
Zhi-hong ZHANG
;
Qian-qian SI
;
Ru-xia WANG
;
Zhi-qiang DENG
;
Yi-bing FAN
;
Liang JIN
;
Jie SUN
;
Chun-hua YANG
Author Information
1. 南昌市疾病预防控制中心,南昌 330038
- Publication Type:Journal Article
- Keywords:
avian influenza virus;
H5N6;
fluorescent quantum dots;
rapid detection
- From:
Chinese Journal of Zoonoses
2025;41(3):243-248,283
- CountryChina
- Language:Chinese
-
Abstract:
In view of the characteristics of H5N6 subtype avian influenza virus(AIV)that it has both high pathogenicity and the risk of cross-species transmission,posing a serious threat to the poultry farming industry and public health security,in order to effectively prevent and control the spread of H5N6 avian influenza,a rapid,sensitive and specific detection technolo-gy was established in this study.The specific monoclonal antibodies against the neuraminidase N6 protein of avian influenza A virus subtype H5N6 were obtained through hybridoma and monoclonal antibody technology.These antibodies were coupled and labeled with carboxyl-functionalized fluorescent quantum dots,along with previously prepared specific antibodies against the hemagglutinin H5 protein.A rapid fluorescence immunochromatographic detection method for the H5N6 subtype of avian influ-enza virus was established according to the principle of double-antibody sandwich immunochromatography.This method a-chieved a detection sensitivity of 1 ng/mL for recombinant hemagglutinin H5 subtype protein and 0.1 ng/mL for recombinant neuraminidase N6 subtype protein.Moreover,the method exhibited no cross-reactivity with other influenza subtypes or patho-gens,such as Newcastle disease(ND),infectious bronchitis(IB),and infectious laryngotracheitis(ILT),thus demonstrating good specificity.The method effectively identified the highly pathogenic avian influenza virus H5 subtype and directly distin-guished the H5N6 subtype with good accuracy.The fluorescent quantum dot immunochromatographic typing detection method established herein met the sensitivity,specificity,and accuracy requirements for H5N6 subtype detection,and can be further used for rapid detection of the H5 and H5N6 subtypes of avian influenza virus.
