Molecular characteristics of the HA genes of H9N2 subtype avian influenza viruses originating from wild birds in wetlands in Fujian
10.3969/j.issn.1002-2694.2025.00.036
- VernacularTitle:福建湿地野鸟源H9N2亚型禽流感病毒HA的分子特征
- Author:
Zhen CHEN
1
;
Chun-hua ZHU
;
Cui-teng CHEN
;
Bin-qiong LIU
;
Guo-zhang CAI
;
Chun-he WAN
;
Yu HUANG
;
Shao-hua SHI
Author Information
1. 福建省农业科学院畜牧兽医研究所,福建省禽病防治重点实验室,福州 350013
- Publication Type:Journal Article
- Keywords:
wetlands in Fujian;
wild bird origin;
H9N2 subtype;
avian influenza virus;
HA
- From:
Chinese Journal of Zoonoses
2025;41(3):227-234
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at evaluating the characteristics of H9N2 subtype avian influenza viruses(AIVs)origina-ting from wild birds in major wetlands in Fujian.Five H9N2 subtype AIVs isolated from fecal samples from wild birds in wet-lands of the Minjiang River,Jiulong River,Sandu Bay,Xinghua Bay,and Quanzhou Bay in Fujian were sequenced.Sequence a-nalysis of the HA genes of the five H9N2 subtype AIVs indicated that the five isolates shared 89.8％-99.4％nucleotide se-quence identity.All five isolates belonged to the same h9.4.2.5c evolutionary branch.The cleavage site motifs of HA were all PSRSSR ↓ GLF,thus indicating molecular characteristics of AIVs with low pathogenicity.The HA proteins of the viruses orig-inating from wild birds bore eight identical potential glycosylation sites,among which the glycosylation site at position 313 was located near the HA protein cleavage site.The 226th amino acid of HA in the receptor binding site was leucine in each virus,thus indicating that HAs of the five H9N2 subtype AIVs had mammalian sialic acid α-2,6 receptor binding affinity.In conclu-sion,the five H9N2 subtype AIVs originating from wild birds in Fujian had low pathogenicity,and the HAs had mammalian sialic acid α-2,6 receptor binding affinity.
