The bioinformatics analysis of miR-144-5p regulated FoxO1 as a po-tential therapeutic target for antibody-mediated rejection in kidney transplantation
10.12092/j.issn.1009-2501.2024.12.006
- VernacularTitle:miR-144-5p调控的FoxO1作为肾移植抗体介导排斥反应潜在治疗靶点的生物信息学分析
- Author:
Yuhui CHAI
1
;
Yunyun YANG
;
Danni QUAN
;
Lingpeng ZHANG
;
Lihong GAO
;
Xuebin WANG
;
Zhuo WANG
Author Information
1. 海军军医大学第一附属医院(上海长海医院)药剂科,上海 200433
- Publication Type:Journal Article
- Keywords:
kidney transplantation;
antibody-me-diated rejection;
miR-144-5p;
FoxO1;
bioinformat-ics analysis
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2024;29(12):1359-1366
- CountryChina
- Language:Chinese
-
Abstract:
AIM:In this study,bioinformatics tech-nology was used to explore the differentially ex-pressed genes in antibody-mediated rejection after renal transplantation,and to screen out the possi-ble mechanisms and potential therapeutic targets of AMR-related miRNAs after renal transplantation,in order to provide new idea for the targeted thera-py of AMR after transplantation.METHODS:The dataset GSE115816 was downloaded from the GEO database,and the differential expression of miR-NAs in stable renal transplantation(SGF)group and the antibody-mediated rejection(AMR)group after renal transplantation was analyzed online by using DESeq 2R software.TargetScan software predicted the related targets of miRNAs,and the differential-ly expressed genes(DEGs)were analyzed through through gene ontology(GO)enrichment analysis and Kyoto gene and genome encyclopedia(KEGG)enrichment analysis,then key genes were screened by String database and Cytoscape,and finally veri-fied by TargetScan online analysis.RESULTS:A total of 10 differentially expressed miRNAs were identi-fied in the AMR group by comparison with the SGF group,with the most significant difference in ex-pression of miR-144-5p.A total of 143 miR-144-5p related targets were predicted by Targetscan soft-ware.GO analysis showed that DEGs were mainly involved in angiogenesis,synaptic signaling,and transcriptional co-activator regulation.KEGG analy-sis showed that DEGs were mainly enriched in the thyroid hormone signaling pathway,human papillo-mavirus infection,and PI3K-AKT signaling pathway.The 10 Hug genes were screened by PPI network.Based on the 6 algorithms in cytoHubba,5 key genes were obtained by taking the intersection of the top 10 Hug genes of each algorithm,which were NCOA2,NCOA1,FOXO1,PAX3,and PPARGC1A.After the literature review,we found that FoxO1 plays an essential role in immune sys-tem diseases and kidney diseases.In our study,we chose FoxO1 as a potential target protein for miR-144-5p.Finally,TargetScan online analysis showed that miR-144-5p has a targeted binding site with the 3'UTR region of FoxO1.CONCLUSION:MiR-144-5p plays an important role in AMR after Kidney transplantation.MiR-144-5p targeting FoxO1 may be a potential therapeutic target and prognostic biomarker for AMR.
