Selection of exosomal microRNA biomarkers for brucellosis diagnosis and construction of a potential miRNA-mRNA regulation network
10.3969/j.issn.1002-2694.2025.00.041
- VernacularTitle:布鲁氏菌病诊断外泌体microRNA生物标志物的筛选和潜在miRNA-mRNA调控网络的构建
- Author:
Jin ZHAO
1
;
Zhi-qiang CHEN
;
Bing-Li WANG
;
Shu-ling LI
;
Xiao-yu ZHU
;
Jin-tong JIA
;
Ye-zi LIU
;
Zhi-wei LI
Author Information
1. 新疆维吾尔自治区人民医院临床检验中心,乌鲁木齐 830001
- Publication Type:Journal Article
- Keywords:
Brucellosis;
Exosomal microRNAs;
biomarker;
miRNA-mRNA regulatory network
- From:
Chinese Journal of Zoonoses
2025;41(3):269-277
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at exploring novel auxiliary diagnostic biomarkers for brucellosis and their potential miR-NA-mRNA regulatory networks.High-throughput sequencing was used to compare miRNA expression differences in serum ex-osomes between patients with brucellosis and healthy controls.Subsequently,RT-qPCR was used to validate the expression of significantly upregulated exosomal miRNAs.The diagnostic value of these miRNAs was assessed with ROC curves,and bioin-formatics analyses were performed to investigate the potential roles of the miRNAs in brucellosis infection.The ROC curve a-nalysis indicated that the area under the curve for exosomal hsa-miR-11400(P＜0.05),hsa-miR-199a-5p(P＜0.05),and hsa-miR-148a-5p(P＜0.05)was 0.79,0.81,and 0.74,respectively.A total of 465 differentially expressed miRNAs and their tar-get genes were predicted,including 25 immune-related target genes,most of which were closely associated with cancer-related proteoglycans,NF-kappa B signaling pathways,and IL-17 signaling pathways.The constructed differentially expressed gene network indicated that the immune genes PLXNA2,IL17RA,PRKCA,CD22,ACVR1B,and CBL might be regulated by hsa-miR-199a-5p and hsa-miR-148a-5p.These findings suggest that exosomal miRNAs might serve as auxiliary diagnostic indicators for brucellosis.Our exosomal miRNA-mRNA regulatory network provides new insights into the pathogenesis and treatment of brucellosis.
