Ultrasonic-assisted Deep Eutectic Solvent Extraction of Ziziphusjujube Polysaccharide and Its Hypoglycemic Activity
10.3870/j.issn.1004-0781.2025.06.015
- VernacularTitle:酸枣多糖低共熔溶剂提取工艺优化及体外降糖活性
- Author:
Yanyan WANG
1
;
Zihao WANG
1
;
Yixuan LI
1
;
Hongkun WANG
1
;
Yonghai MENG
1
;
Chunmei ZHAI
1
Author Information
1. 黑龙江中医药大学药学院中药药理教研室,哈尔滨 150040
- Publication Type:Journal Article
- Keywords:
Ziziphusjujube polysaccharide;
Box-Behnken response surface methodology;
Deep eutectic solvent
- From:
Herald of Medicine
2025;44(6):928-933
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the extraction process of Ziziphusjujube polysaccharides and study their hypoglycemic effect in vitro.Methods Using deep eutectic solvent as the extraction solvent,the optimal extraction condition for Ziziphusjujube polysaccharides was selected using single factor experiments and Box Behnken experiments.The selected factors were water content,solid-liquid ratio,and ultrasound time.At the same time,the content of Ziziphusjujube polysaccharideswas determined,and the in vitro hypoglycemic effect of polysaccharides and the hypoglycemic ability of different parts of Ziziphusjujube were investigated.Results After Box-Behnken response surface optimization,the optimal extraction condition for Ziziphusjujube polysaccharides was as follows:after ultrasonic treatment for 41.68 minutes,solid-to liquid ratio of 1∶20,and the water content in the deep eutectic solvent was 19.96%,under these conditions,the Ziziphusjujube polysaccharide content reach(571.31±3.84)mg·g-1 α-amylase inhibition rate of Ziziphusjujube polysaccharides was 76.08%,the content of polysaccharide in whole fruit of Ziziphusjujube was the highest,while the pulp exhibited the strongest hypoglycemic activity.Conclusion The optimized process can providea areference for the development in industry and clinical application of Ziziphusjujube.
