Mechanistic study of Yigan Fupi decoction-mediated repair of the intestinal barrier and reduction of IBS sensitivity through regulation of the PKA/PKC-CREB pathway
10.3969/j.issn.1005-4847.2025.04.005
- VernacularTitle:抑肝扶脾汤调控PKA/PKC-CREB通路修复肠道屏障降低IBS敏感性的机制研究
- Author:
Yu CHEN
1
;
Jiajun SHI
;
Danting FU
;
Qinqin YANG
;
Rui FU
;
Jiajie ZHU
;
Mingjin ZHU
;
Xinying LIU
;
Mingxian CHEN
Author Information
1. 浙江省中医药研究院,杭州 310007
- Publication Type:Journal Article
- Keywords:
Yigan Fupi decoction;
intestinal barrier;
IBS sensitivity;
PKA/PKC;
CREB
- From:
Acta Laboratorium Animalis Scientia Sinica
2025;33(4):512-521
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the therapeutic effects of Yigan Fupi decoction(YGFP)on irritable bowel syndrome(IBS)and its mechanism of action in repairing the intestinal barrier and reducing IBS sensitivity through the PKA/PKC-CREB pathway.Methods Baby rats separated from their mother were randomly divided into a model control(M)and a YGFP group,while baby rats without maternal separation were used as a normal control(N)group.The YGFP group was given YGFP for 4 weeks.Abdominal withdrawal reflux was used to evaluate intestinal sensitivity.Liquid chromatography tandem mass spectrometry and ELISA were used to detect bile acid metabolite concentrations and serum levels of interleukin(IL)-6 and CXCL1,respectively.HE staining was used to observe pathological changes in the colon,and Western Blot and immunohistochemistry were used to analyze the relative protein expression levels of PKA,PKC,CREB,5HT2AR,5-HT7R,ZO-1,and Claudin 1.Results Compared with the normal control group,the M group showed a significantly decreased visceral pain threshold,significantly increased levels of total bile acid metabolites,IL-6,and CXCL1,significantly increased relative expression of PKA,PKC,CREB,5HT2AR,and 5-HT7R,and significantly decreased relative expression of ZO-1 and Claudin 1.Compared with the M group,the YGFP group showed a significantly increased visceral pain threshold,significantly reduced levels of total bile acid metabolites,IL-6,and CXCL1,significantly reduced relative expression of PKA,PKC,CREB,5HT2AR,and 5-HT7R,and increased relative expression of ZO-1 and Claudin 1.Conclusions YGFP effectively improved IBS through a mechanism that may involve repair of the intestinal barrier and reduced sensitivity through the PKA/PKC-CREB pathway.
