Impact of lupeol on periodontal tissue injury in rats with periodontitis by regulating RhoA/ROCK signa-ling pathway
10.3969/j.issn.1001-3733.2025.03.004
- VernacularTitle:羽扇豆醇调节RhoA/ROCK信号通路对牙周炎大鼠牙周组织损伤的影响
- Author:
Daorong WANG
1
;
Chengli NI
;
Ao DING
Author Information
1. 230001,合肥市第二人民医院(安徽医科大学附属合肥医院)口腔科
- Publication Type:Journal Article
- Keywords:
Lupeol;
Periodontitis;
Osteoclast;
Periodontal tissue
- From:
Journal of Practical Stomatology
2025;41(3):321-327
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the impact of lupeol on periodontal tissue injury in periodontitis rats by regulating Ras homolog gene family member A(RhoA)/Rho associated coiled coil containing protein kinase(ROCK)signal pathway.Methods:SD rats were randomly divided into control group(NC group),Model group,Lupeol of low(Lupeol-L group,16.67 μg/kg),medium(Lupeol-M group,33.34 μg/kg)and high-dose(Lupeol-H group,66.68 μg/kg)groups,vitamin C(VC)group(100 mg/kg),Rhosin(RhoA inhibitor)group(40 mg/kg)and Lupeol-H+Rhosin group(66.68 μg/kg+40 mg/kg)groups(n=18).Except NC group,the rats in other groups were used to establish periodontitis models.After modeling,the drugs were administered to the mice of the groups respectively,once a day for 21 days.Serum levels of IL-1β and TNF-α were detected by ELISA.Alveolar bone resorption in rats was detected by methylene blue staining.HE staining was used to detect the pathological changes of periodontal tissues.The expres-sion of OPG protein in periodontal tissue was detected by immunohistochemistry.TRAP staining was used to detect the osteoclasts in rat periodontal tissue,and the expression of OPG,RANKL,RhoA,ROCK1 and ROCK2 proteins in periodontal tissue was detected by Western blot.Results:Compared with NC group,pathological injury of periodontal tissue was serious,TNF-α and IL-1β levels,the distance from alveolar crest to enamel cementum junction(CEJ),osteoclasts number and RANKL protein increased,the num-ber of osteoblasts,and the expression of OPG,RhoA,ROCK1 and ROCK2 proteins decreased in model group(P＜0.05).Compared with model group,pathological injury of periodontal tissue in all Lupeol groups and VC group was reduced,the levels of TNF-α and IL-1β,the distance from alveolar crest to CEJ,osteoclasts number and RANKL protein decreased,the number of osteoblasts,and the expression of OPG,RhoA,ROCK1 and ROCK2 proteins increased,the cor-responding indexes of Rhosin group showed the opposite trend(P＜0.05).Rhosin reversed improvement effect of high dose Lupeol on periodontal tissue injury in rats with periodontitis.Conclusion:Lupeol may reduce periodontal tissue injury in rats with peri-odontitis by activating RhoA/ROCK signaling pathway.
