Functional decellularized porcine cartilage particles promote the repair of articular osteochondral de-fects
10.3969/j.issn.1001-3733.2025.03.002
- VernacularTitle:功能化脱细胞猪软骨颗粒促进关节骨软骨缺损修复
- Author:
Peixue ZHUANG
1
;
Pingping YUAN
;
Na WEI
;
Xinchi ZHANG
;
Yujiao WANG
;
Wei WU
Author Information
1. 710032 西安,口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔疾病临床医学研究中心,空军军医大学口腔医院口腔颌面外科
- Publication Type:Journal Article
- Keywords:
Decellularized extracellular matrix;
Articular defects;
Biomaterial;
Endogenous regeneration
- From:
Journal of Practical Stomatology
2025;41(3):305-313
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:To evaluate the effectiveness of functionalized decellularized extracellular matrix(F-dECM)prepared from porcine auricular cartilage or porcine rib cartilage in repairing xenogeneic articular osteochondral defects.Methods:The por-cine auricular cartilage and rib cartilage were crushed and decellularized.Heparin modification and multi-factor loading were a-chieved through amide bond reaction.The physical characteristics of the particles were characterized by SEM observation and mer-cury intrusion.The chemical characteristics of the particles were characterized by tissue section staining and kit detection.The rabbit knee joint full-thickness defect models were established and implanted by different particles respectively.After 6 weeks and 12 weeks of operation,the samples were taken for gross observation,CT scanning and tissue sectioning to comprehensively evaluate the repair effect.Results:After heparin modification,the glycosaminoglycan lost during the decellularization process was replen-ished.Both heparinized auricular cartilage and costal cartilage presented a highly developed porous structure.Among them,the porosity and pore diameter of costal cartilage were both lower than those of auricular cartilage(P＜0.05).During the observation periods of 6 weeks and 12 weeks,the porcine costal cartilage F-dECM implantation group was superior to other experimental groups in knee joint repair effects(P＜0.05).Conclusion:Costal-derived F-dECM is a joint osteochondral defect repair material with application potential.
