Study on the effect of mild photothermal effect of Au nanorods on the osteogenic properties of MC3T3-E1 osteoblasts
10.3969/j.issn.1001-3733.2025.03.003
- VernacularTitle:金纳米棒的温和光热效应对MC3T3-E1成骨性能的研究
- Author:
Peibo YAN
1
;
Wen SONG
;
Zhao MU
;
Jinchan LIU
;
Zhiwei MA
Author Information
1. 710032 西安,口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔疾病国际联合研究中心,空军军医大学口腔医院牙周病科
- Publication Type:Journal Article
- Keywords:
Au nanorods;
BSA;
Mild photothermal;
MC3T3-E1;
Osteogenesis
- From:
Journal of Practical Stomatology
2025;41(3):314-320
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:To investigate the influence of the mild photothermal effect of Au nanorods on the osteogenic differentiation of MC3T3-E1 osteoblast cells.Methods:Surface modification of Au nanorods was performed with bovine serum albumin(BSA)coating.The photothermal effect,photothermal stability and characteristics of AuNRs@CTAB and AuNRs@BSA were analyzed u-sing scanning electron microscopy(SEM),transmission electron microscopy(TEM),and a near-infrared laser.The biocompatibili-ty of AuNRs@BSA was evaluated using live/dead staining and the CCK-8 assay.The influence of the mild photothermal effect of AuNRs@BSA on the osteogenic differentiation of MC3T3-E1 was assessed by ALP staining,alizarin red staining,and semi-quanti-tative analysis.Results:Biocompatible and near-infrared light-responsive AuNRs@BSA were prepared.In vitro experiments dem-onstrated that the mild photothermal effect of AuNRs@BSA increased the ALP activity and mineralization ability of MC3T3-E1(P＜0.000 1).Conclusion:The mild photothermal effect of AuNRs@BSA can promote the osteogenic differentiation of MC3T3-E1 cells.
