Effect of tumor necrosis factor receptor-associated factor 6 on pro-inflammatory factors after Kaposi's sarcoma-associated herpesvirus infection
10.12138/j.issn.1671-9638.20246434
- VernacularTitle:肿瘤坏死因子受体相关因子6对卡波西肉瘤相关疱疹病毒感染后促炎因子的影响
- Author:
Ge WU
1
;
Rong-jiong ZHENG
;
Ke-jun PAN
;
Dan HAN
;
Xiao-bo LU
Author Information
1. 新疆医科大学第一附属医院肿瘤二科,新疆乌鲁木齐 830054
- Publication Type:Journal Article
- Keywords:
tumor necrosis factor receptor-associated factor 6;
Kaposi's sarcoma;
Kaposi's sarcoma-associated her-pesvirus;
KSHV;
pro-inflammatory factor
- From:
Chinese Journal of Infection Control
2024;23(12):1471-1476
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of pro-inflammatory factors including interleukin(IL)-1a,IL-1β,IL-6 and IL-8 after silencing and over-expressing of tumor necrosis factor receptor-associated factor 6(TRAF6)in vitro,explore the effect of TRAF6 on pro-inflammatory factors after Kaposi's sarcoma-associated herpesvirus(KSHV)infection,and provide new ideas for the study of the pathogenesis of KSHV.Methods The silence and over-expression TRAF6 models were established using KSHV(+)iSLK cell line,and the transfection level was de-tected by real-time fluorescence quantitative polymerase chain reaction(RT-PCR).After 48 and 72 hours of cultiva-tion of cells,the expression levels of pro-inflammatory factors after silencing and over-expressing TRAF6 were de-tected by enzyme-linked immunosorbent assay(ELISA)and compared.Results TRAF6-silence(siTRAF6)and TRAF6-over-expression(TRAF6-OE)cell models were established successfully.ELISA results showed that pro-inflammatory factors IL-1α,IL-1β,IL-6,and IL-8 increased in varying degrees after silencing TRAF6,while these pro-inflammatory factors decreased in varying degrees after TRAF6 over-expression,IL-6 and IL-8 levels changed remarkablely,suggesting that TRAF6 had a certain inhibitory effect on pro-inflammatory factors and played an im-portant role in the signaling pathway of pro-inflammatory effects after KSHV infection.Conclusion TRAF6 has a certain inhibitory effect on pro-inflammatory factors and is expected to serve as a potential target to inhibit the pro-gression of Kaposi's sarcoma.
