Characteristics of structure and functions of Rv0037c protein of Mycobac-terium tuberculosis
10.12138/j.issn.1671-9638.20246296
- VernacularTitle:结核分枝杆菌蛋白Rv0037c的结构和功能特征
- Author:
Xue-jiao JING
1
;
Li SONG
;
Chang-xin WU
Author Information
1. 山西大学生物医学研究院,山西 太原 030006
- Publication Type:Journal Article
- Keywords:
Rv0037c protein;
bioinformatics;
biological function;
Mycobacterium tuberculosis;
Mycobacterium smegmatis;
drug resistance
- From:
Chinese Journal of Infection Control
2024;23(12):1463-1470
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the structure and functions of Rv0037c protein,a member of major facilitator su-perfamily(MFS)proteins of Mycobacterium tuberculosis.Methods Bioinformatics analysis on Rv0037c protein was performed with TMHMM Server v.2.0,Swiss-model and DNAMAN software.The PMV261-Rv0037c overex-pressed strain was constructed in Mycobacterium smegmatis,pMV261/Msm control strain was obtained,its biolo-gical function was analyzed.Results Rv0037c protein contained 11 transmembrance domains,the C/N domain con-sisted of"3+2"helical bundles,which was highly conservative in genus Mycobacterium.The growth kinetics curves of the overexpressed strain and the control strain almost completely overlapped,and there was no statistical difference in growth between the overexpressed strain and the control strain(P＞0.05).The OD600 of the control strain was 2.567±0.162,while that of the overexpressed strain was 2.419±0.456,there was no statistical diffe-rence in biofilm growth between the two strains(P＞0.05).The accumulation of ethidium bromide(EB)in overex-pressed strain was lower than that in control strain,difference was statistically significant(P＜0.001).There was no statistically significant difference in the minimum inhibitory concentration(MIC)of two bacterial strains against various drugs(both P＞0.05).Conclusion The Rv0037c protein is a special member of the Mycobacterium MFS family proteins and has a very conserved structure.Overexpression of Rv0037c has no effect on the growth,colony morphology,sliding ability,biofilm formation,and MIC of Mycobacterium smegmatis,but can significantly reduce the accumulation of EB.
