Selection of fecal antigen diagnostic markers for Echinococcus granulosus
10.16303/j.cnki.1005-4545.2025.04.13
- VernacularTitle:犬细粒棘球绦虫粪抗原诊断标志物筛选
- Author:
Wanli BAN
1
;
Shuai LIU
;
Bingjie WANG
;
Kamali WULIJIANG
;
Xingyu PAN
;
Yan WANG
;
Talipuhan GU-LIZHATI
;
Jing XU
;
Teliewuhan MUNILA
;
Zhuangzhi ZHANG
;
Li ZHAO
Author Information
1. 新疆畜牧科学院兽医研究所 新疆畜牧科学院动物临床医学研究中心/国家动物包虫病参考实验室,新疆乌鲁木齐 830013
- Publication Type:Journal Article
- Keywords:
Echinococcus granulosus;
monoclonal antibody;
antigen;
unique peptides;
diagnostic bio-markers
- From:
Chinese Journal of Veterinary Science
2025;45(4):717-723
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to screen the diagnostic biomarkers for fecal antigen of Echinococcus granulosus(E.granulosus)in dogs with high specificity and sensitivity.The sheep-derived EgPSC artificially infected dogs were collected,and the negative and positive fecal samples of dogs with E.granulosus were prepared by arecoline hydrobromide leakage method.Polyclonal antibody,negative fecal antigen-polyclonal antibody conjugates and positive fecal antigen-polyclonal antibody conju-gates were purified by ammonium sulfate precipitation and affinity chromatography,three groups of samples were detected by ELISA and Western blot,LC-MS/MS and bioinformatics analysis were performed on the three groups of samples.The positive fecal antigen-polyclonal antibody con-jugate was used as the treatment group,the polyclonal antibody and the negative fecal antigen-polyclonal antibody conjugates were used as the control groups to screen the unique peptides of the treatment group.ELISA and Western blot showed that only the positive fecal antigen-polyclonal antibody conjugates were positive.According to LC-MS/MS and bioinformatics analysis,11 unique peptides were screened out only in the treatment group.Among them,3 proteins were related to E.granulosus,namely dysferlin,integrator complex 9 and diagnostic antigen gp50,which were mem-brane-associated proteins,INT complex components and diagnostic antigens.This study has pre-liminarily screened out three candidate canine E.granulosus fecal antigen diagnostic markers,pro-viding a reference for further exploration of diagnostic standards for E.granulosus,screening of echinococcosis target genes,and vaccine development.
