Isolation,identification and whole genome sequencing analysis of a bovine-derived type A Pasteurella multocida strain
10.16303/j.cnki.1005-4545.2025.04.07
- VernacularTitle:1株牛源A型多杀性巴氏杆菌的分离鉴定及全基因组测序分析
- Author:
Yaping WANG
1
;
Shixing WANG
1
;
Xiwen FAN
1
;
Xiaojia KONG
1
;
Zhen WANG
1
Author Information
1. 北京农学院动物科学技术学院兽医学(中兽医)北京市重点实验室,北京 102206
- Publication Type:Journal Article
- Keywords:
Pasteurella multocida;
bovine origin;
whole-genome sequencing;
drug resistance;
virulence
- From:
Chinese Journal of Veterinary Science
2025;45(4):668-677
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to isolate and identify the Pasteurella multocida(Pm)that caused re-spiratory infections in cattle at a farm in Beijing,and to clarify the biological characteristics of the pathogen,including its serotype,drug resistance,pathogenicity,and genomic features.For this pur-pose,nasopharyngeal swabs and tissue samples were collected from an infected cow for bacterial i-solation and purification,16S rRNA gene amplification,capsular serotyping,virulence gene detec-tion,and whole-genome sequencing of the isolated strain.The results showed that after bacterial i-solation and purification,biochemical tests,and PCR identification,a type A P.multocida strain named"Pm WZ01"was identified.The virulence gene amplification revealed that the Pm WZ01 ge-nome contained the tadD,nanH,ompA,exbB,exbD,and fur genes.Whole-genome sequencing determined that the Pm WZ01 strain had a genome size of 2 383 021 bp with a GC content of 40.28％,containing 56 tRNAs,19 rRNAs,155 pseudogenes,three genomic islands,five prophages,and 55 carbohydrate-active enzyme genes.CARD annotation analysis found one PBP3 resistance gene and two EF-Tu resistance genes.VFDB and PHI-base annotation analysis identified 582 viru-lence factor-related genes and 888 phenotypic mutation genes,etc.Phylogenetic tree analysis revealed that the Pm WZ01 strain is most closely related to the Pm-3 strain(CP014618.1)from Changchun,China,the P1933 strain(CP132898.1)from the UK,and the HB01 strain(CP006976.1)from Hubei,China,belonging to the same clade.This study completed the isolation and identifica-tion of a type A P.multocida strain as well as the analysis of its biological characteristics and whole-genome sequencing,providing a theoretical basis for the prevention and control research of bovine type A P.multocida disease.
