Establishment and preliminary application of a fluorescent recombinase-aided am-plification method for detection of porcine epidemic diarrhea virus
10.16303/j.cnki.1005-4545.2025.04.03
- VernacularTitle:检测PEDV核酸的荧光RAA方法的建立及初步应用
- Author:
Hao MU
1
;
Mingni LIU
;
Lindan LYU
;
Shaomei LI
;
Guoyang XU
;
Liu YANG
Author Information
1. 重庆市畜牧科学院,重庆荣昌 402460;生猪技术创新中心(重庆),重庆荣昌 402460
- Publication Type:Journal Article
- Keywords:
PEDV;
fluorescent RAA;
M gene;
free of nucleic acid extraction
- From:
Chinese Journal of Veterinary Science
2025;45(4):640-647
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to develop a recombinase aided amplification(RAA)technology to de-tect porcine epidemic diarrhea virus(PEDV).A set of RAA primers and probes with high amplifi-cation efficiency and specificity was designed,specifically targeting the M gene.The amplification process was monitored in real-time using a fluorescent constant temperature detector to facilitate the rapid,sensitive,and specific detection of PEDV nucleic acids.The results showed that the es-tablished method exhibited excellent sensitivity,with a minimum detection limit of 8.86 × 101 copies/μL.Furthermore,the detection method has good specificity and reproducibility,with no cross-reactions observed with other porcine viruses such as transmissible gastroenteritis virus(TGE),porcine coronavirus,and porcine circovirus.The method also showed clear amplification curves at constant temperatures ranging from 37.0 to 41 ℃,highlighting its good temperature a-daptability.The establishment of fluorescent RAA technology for PEDV detection method provides a method for on-site rapid detection of PEDV.
