Phylogenetic analysis of adenovirus and arenavirus in rodents from coastal cities in Fujian Province
10.3969/j.issn.1002-2694.2024.00.166
- VernacularTitle:福建沿海城市鼠腺病毒和沙粒病毒调查及进化分析
- Author:
Zhi-wei ZENG
1
;
Jing LIU
1
;
Guo-ying XU
1
;
Teng-wei HAN
1
;
Jia-xiong WANG
1
;
Shu-heng ZHOU
1
;
Ling-qiong HUANG
1
;
Fang-zhen XIAO
1
Author Information
1. 福建省疾病预防控制中心,福建省人兽共患病研究重点实验室,福州 350012
- Publication Type:Journal Article
- Keywords:
murine adenovirus;
arenavirus;
rodent;
phy-logenetic analysis
- From:
Chinese Journal of Zoonoses
2024;40(11):1073-1078
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at exploring the infections and viral genetic characteristics in rodents from coastal cities in Fujian Province.Intestinal tissue samples were obtained from rodents in Ningde,Fuzhou,Quanzhou,and Zhangzhou city.The adenovirus DNA polymerase(DPOL)gene was analyzed by nested PCR,and the large(L)segment gene of arenavirus was as-sessed with RT-PCR.Homology and genetic characteristics were analyzed in bioinformatics software.A total of 152 rodents were captured and showed a murine adenovirus infection rate of 4.61％.The murine adenovirus infection rate was 5.26％in wild rodents and 4.21％in domestic rodents.Murine adenoviruses were detected in four rodent species:Rattus sladeni,Rattus nor-vegicus,Rattus tanezumi,and Rattus losea.This was the first time that murine adenovirus has been detected in Rattus slade-ni.Analysis of relevant factors revealed no significant differences in murine adenovirus infection rates by rodent species,sex,age,and habitat.Sequence analysis indicated that the adenoviruses infecting rodents in coastal cities of Fujian were Murine ade-novirus 2 and Murine adenovirus 3.A significant difference was observed in the sequences of murine adenoviruses with respect to those in other regions,with nucleic acid sequence identity ranging from 72.25％to 91.01％.No arenavirus was detected in any rodent specimens.Adenovirus infections were found in rodents in coastal cities of Fujian Province,but no arenavirus infec-tions were found.This study provides useful information to support further research on murine adenovirus and arenavirus in Fujian Province.
