Establishment and application of a colloidal gold immunochromatographic test strip assay for detection of caprine enterovirus
10.16303/j.cnki.1005-4545.2025.04.06
- VernacularTitle:羊肠道病毒胶体金免疫层析试纸条检测方法的建立及初步应用
- Author:
Xuebo ZHENG
1
;
Xuyuan CUI
;
Naitian YAN
;
Qianying WANG
;
Hongwei LI
;
Lihui CHEN
;
Wenjie GONG
;
Xinping WANG
Author Information
1. 吉林大学动物医学学院,吉林长春 130062
- Publication Type:Journal Article
- Keywords:
caprine enterovirus;
double antibody sandwich;
colloidal gold chromatography test strip;
VP1 protein
- From:
Chinese Journal of Veterinary Science
2025;45(4):662-667
- CountryChina
- Language:Chinese
-
Abstract:
To establish an immunochromatographic method for rapid detection of caprine enterovir-us(CEV),the monoclonal antibody against CEV VP1 protein was used as gold-labeled monoclonal antibodies,and the purified rabbit-derived polyclonal antibody of CEV-VP1 and sheep anti-mouse IgG were used as the detection line and quality control line,respectively.The colloidal gold immu-nochromatographic test strips for CEV were prepared according to the principle of double antibody sandwich,evaluated,and applied for clinical specimen detection.The results showed that the meth-od specifically recognized CEV without cross-reaction with bovine enterovirus and bovine viral di-arrhea virus.The minimum detection limit of the method was 102.49 TCID50/mL and had good re-producibility.The prepared test strips had a shelf life of three months kept at 4 ℃.Detection of clin-ical samples using the immunochromatographic test strips showed 100％coincidence rate with RT-PCR method.In conclusion,the colloidal gold immunochromatographic test strips for detection of the emerging CEV with good specificity,sensitivity and repeatability,which provides a new techni-cal means easily used for the rapid detection/diagnosis and epidemiological investigation on CEV infection.
