Effects of type and abundance of sialic acid receptors on susceptibility and viral ti-ter of different strains of Newcastle disease virus
- VernacularTitle:唾液酸受体类型和表达丰度对新城疫病毒不同毒株易感性和病毒滴度的影响
- Author:
Bingmei DONG
1
;
Wenya XU
1
;
Lili SONG
1
;
Yanling XING
1
;
Mengran LI
1
;
Ziye LI
1
;
Xueqing WEN
1
;
Jingran ZHANG
1
;
Jingyu ZHANG
1
;
Qiyuan NING
1
Author Information
- Publication Type:Journal Article
- Keywords: sialic acid receptor; Newcastle disease virus; infectivity; viral titer
- From: Chinese Journal of Veterinary Science 2025;45(4):656-661
- CountryChina
- Language:Chinese
- Abstract: The receptors of Newcastle disease virus(NDV)are sialic acid receptors that mainly in-clude neu5ac-α-2,3gal-β-1,4Glc(SAα2,3Gal)and neu5ac-2-s-α-2,6Gal10Me(SAα2,6Gal).The distribution and abundance of the two receptors in host cells have important effects on virus sus-ceptibility and intracellular proliferation.In order to further explore the effects of sialic acid recep-tors on susceptibility and proliferation characteristics of NDV different strains,the expression lev-els of SAα2,3Gal and SAα2,6Gal receptors on BHK-21 cell membrane were adjusted by overex-pression and RNAi assays,and the TCID50 values were determined after different BHK-21 cells were inoculated with NDV strains Ⅰ and LaSota.The results suggested that NDV strain LaSota preferentially binds to SAα2,6Gal and strain Ⅰ selectively binds to SAα2,3Gal receptor.Further-more,the viral titers of NDV strains LaSota and Ⅰ in cell culture were positively correlated with the expression levels of SAα2,6Gal and SAα2,3Gal receptors on host cell membrane respectively.In conclusion,our studies provide an understanding of the relationship between infectivity of NDV different strains and receptor types of host cell,and provide a method to increase viral titer of NDV for cell-based vaccine production.
