Effect of pinocembrin on the malignant biological behavior of gastric can-cer cells by regulating the RhoA/ROCK signaling pathway
10.3969/j.issn.1009-9905.2025.08.003
- VernacularTitle:松属素调节RhoA/ROCK信号通路对胃癌细胞恶性生物学行为的影响
- Author:
Rong PENG
1
;
Ze-min ZHANG
;
Zhi-qing WANG
;
Bin LI
;
Li-ping QING
;
Jin-xing WANG
Author Information
1. 攀枝花市中心医院 放射影像科(四川 攀枝花 617067)
- Publication Type:Journal Article
- Keywords:
Gastric neoplasms;
Pinocembrin;
Ras homolog gene family member A/Rho-associated coiled-coil con-taining kinase signaling pathway;
Proliferation;
Migration;
Invasion;
Epithelial mesenchymal transition
- From:
Chinese Journal of Current Advances in General Surgery
2025;28(8):601-606
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Exploring the effect of Pinocembrin(Pino)regulating the Ras homolog gene family member A/Rho associated with curly helix binding protein kinase(RhoA/ROCK)signaling pathway of Ras homologous gene family members on the malignant biological behavior of gastric cancer cells.Methods:Cultivate human gastric cancer cells MGC803 with different concentrations of Pino(0～240μmol/L),detect cell survival rate using CCK-8 method,and screen for the optimal drug concentration.MGC803 cells were rseparated into MGC803 group(Control group),Pino-L group,Pino-M group,Pino-H group,and Pino-H+RhoA agonist CN03 group.The clone formation experiment was applied to detect the number of clones formed of cells in each group.Assessment of cell apoptosis using flow cytometry.Tran-swell invasion and migration experiments were used to detect the number of cells undergoing migration and invasion in each group;Detection of RhoA/ROCK signaling pathway and expression of epithelial mesenchymal transition related proteins in MGC803 cells using Western blot method.Results:Compared with the MGC803 group,the cell survival rate,clone formation number,migration cell number,and invasion cell number were all reduced in the Pino-L group,Pino-M group,and Pino-H group,and RhoA was also present in the cells,ROCK2,The expression levels of vimentin and N-cadherin gradually decreased(P<0.05),while the apoptosis rate and E-cadherin expression level gradually in-creased(P<0.05).The Pino-H+CN03 group reversed the trend of changes in the above indicators).Conclusion:Pino can prevent malignant biological behavior of gastric cancer cells,which may be related to the inhibition of the RhoA/ROCK signaling pathway.
