Molecular epidemiological characteristics of Brucella in Shanxi Province from 2020 to 2023
10.3969/j.issn.1002-2694.2024.00.163
- VernacularTitle:2020-2023年山西省布鲁氏菌分子流行病学特征分析
- Author:
Yang WANG
1
;
Su-xia YAO
1
;
Rui-e HAO
1
;
Ji-ting HAN
1
;
Qiu-xiang ZHANG
1
;
Hong-xia YANG
1
Author Information
1. 山西省疾病预防控制中心,重点传染性疾病预防与诊治山西省重点实验室,太原 030012
- Publication Type:Journal Article
- Keywords:
Brucella;
molecular subtyping;
MLVA;
cgMLST
- From:
Chinese Journal of Zoonoses
2024;40(11):1031-1035
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at analyzing the molecular epidemiological characteristics of Brucella in Shanxi Province from 2020 to 2023.A total of 479 clinical Brucella strains were identified in Shanxi Province from 2020 to 2023.The strains were preliminarily identified through biochemical methods.The genome was extracted for MLVA-16 typing and whole genome sequencing.MLVA-16 clustering results indicated 50.5％to 100％strain similarity.The strains were typed into 204 VNTR types by MLVA-16,whereas the panel 2B locus showed polymorphism.The clustering results indicated 11 VNTR types with more than five strains.Whole genome sequencing was performed for VNTRS types in large clusters.cgMLST clustering indica-ted similar clustering of strains.Brucella was effectively distinguished through MLVA typing.The phylogenetic relationship and epidemiological characteristics of strains can be distinguished more effectively by cgMLST.Specific Brucella genotypes were transmitted in Shanxi Province from 2020 to 2023.
