Isolation and identification of porcine pathogenic Escherichia coli and detection of virulence genes and analysis of drug resistance
10.16303/j.cnki.1005-4545.2025.05.08
- VernacularTitle:猪源致病性大肠杆菌分离鉴定及毒力基因检测和耐药性分析
- Author:
Shuoqi LIU
1
;
Ying LIU
;
Ziwei MENG
;
Jingwen ZHANG
;
Jinghui FAN
;
Yuzhu ZUO
Author Information
1. 河北农业大学 动物医学院,河北 保定 071001
- Publication Type:Journal Article
- Keywords:
Escherichia coli;
virulence genes;
drug resistance;
biofilm;
phylogenetic clustering;
piglet
- From:
Chinese Journal of Veterinary Science
2025;45(5):940-947
- CountryChina
- Language:Chinese
-
Abstract:
To understand the pathogenicity and drug resistance of swine-derived E.coli and its bio-logical characteristics in some areas in Hebei,E.coli was isolated and identified from diarrheal fe-ces of piglets collected from swine farms,and the isolated strains were subjected to drug sensitivity test,detection of the ability to form biofilm,pathogenicity test,virulence gene test,drug resistance gene test,and identification of phylogenetic subgroups.The results showed that a total of 35 patho-genic E.coli strains were isolated from the feces of diarrheic piglets,and most of the isolates were multidrug-resistant,and were resistant to at least three antibiotics,including amoxicillin(88.57％),ampicillin(88.57％),doxycycline(88.75％),sulfisoxazole(77.17％),lincomycin(100％),and chloramphenicol(100％);the isolates were severely resistant.The isolates all carried virulence genes,with a total of five virulence genes detected,namely,EAST1(77.14％),eaeA(17.14％),stx2e(5.71％),LT(2.86％),and STb(2.86％),and the isolates also carried multi-re-sistance genes,with a total of five virulence genes detected,namely,bla TEM-1(65.71％),bla CTX-M(20.00％),tetA(82.86％),tetB(14.29％),aadA2(17.14％),aac(6')-Ib(14.29％),qnrS(17.14％),sul 1(40.00％),sul2(34.29％),and floR(60.00％);the phylogenetic grouping showed that the isolates had a high proportion of group B1 and group A;and all 35 isolates showed differ-ent pathogenicity after infection of mice.This study provides a reference for the selection of effec-tive therapeutic drugs and the development of prevention and control programs for swine-origin pathogenic E.coli in Hebei Province.
