Effect of TPCK trypsin on proliferation of porcine epidemic diarrhea virus in Vero cells
10.16303/j.cnki.1005-4545.2025.05.05
- VernacularTitle:TPCK胰酶对猪流行性腹泻病毒在Vero细胞上增殖的影响
- Author:
Damei ZHANG
1
;
Liu YANG
;
Guangliang GAO
;
Shaomei LI
;
Jie LUO
;
Lizhi FU
;
Yuandi YU
;
Guoyang XU
Author Information
1. 重庆市畜牧科学院,重庆 402460;国家生猪技术创新中心,重庆 402460;重庆市兽用生物制品工程技术研究中心,重庆 402460
- Publication Type:Journal Article
- Keywords:
TPCK trypsin;
porcine epidemic diarrhea virus;
Vero cell;
proliferation;
invasion
- From:
Chinese Journal of Veterinary Science
2025;45(5):919-925
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study is to investigate the effect of TPCK trypsin on the proliferation pattern of porcine epidemic diarrhea virus in Vero cells.The TPCK trypsin and conventional tryp-sin were added for virus proliferation,and RT-qPCR technology was used to analyze the changes in virus adsorption and invasion in Vero cells.The replication ability of porcine epidemic diarrhea vi-rus in Vero cells was explored through growth curve drawing,IFA identification,and cell activity detection.The results showed that the optimal concentrations of TPCK trypsin and conventional trypsin were 1 mg/L and 6 mg/L,respectively.The virus showed a decreasing trend with the pro-longation of TPCK trypsin and conventional trypsin pretreatment time.Adding different pancreatic enzymes during the virus proliferation process did not promote the virus invasion in Vero cells.Af-ter 4 h of invasion,the virus particles of each group gradually increased.By plotting the growth curve,it was found that the virus content of the TPCK trypsin group reached its highest level at 24 h(lgTCID50=(6.30±0.14)/0.1 mL),followed by a decreasing trend at 36 h,and the fluorescence intensity produced at 24 h was higher than that of conventional trypsin.In summary,TPCK trypsin has a better promoting effect on the proliferation of porcine epidemic diarrhea virus in Vero cells.It provided theoretical basis for further research on the mechanism of TPCK trypsin affecting porcine epidemic diarrhea virus proliferation,and also provided data support for the isola-tion and purification of porcine epidemic diarrhea virus epidemic strains.
