Expression of epitopes of spike protein of porcine epidemic diarrhea virus and screening of its nanobody
10.16303/j.cnki.1005-4545.2025.05.04
- VernacularTitle:猪流行性腹泻病毒纤突蛋白抗原表位的表达及其纳米抗体的筛选
- Author:
Xiangyun HU
1
;
Shaomei CHEN
1
;
Zeyi XUAN
1
;
Menghe LUO
1
;
Rui PAN
1
;
Kai YANG
1
;
Yulian XI
1
;
Yanhong CAO
1
Author Information
1. 广西农业职业技术大学畜牧研究院,广西南宁 530007
- Publication Type:Journal Article
- Keywords:
porcine epidemic diarrhea virus;
spike protein;
epitopes;
nanobody;
reactivity
- From:
Chinese Journal of Veterinary Science
2025;45(5):913-918
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to prepare nanobodies for the epitopes of spike protein(S)of porcine ep-idemic diarrhea virus(PEDV),and verify its reactivity.The expression vector pCZN1-SN was con-structed by the prokaryotic expression system,and SN fragment was expressed in prokaryotic ex-pression and purified by Ni-NTA chromatography column.The SN nanobodies were displayed u-sing phage display technology and screened from the natural nanobody library.The results showed that SN fragments with a size of 26 kDa were obtained by prokaryotic induction,and the specific nanobodies were obtained through three rounds screening by phage display technology.One strain with the best reactivity was selected for prokaryotic expression and purified.The nanobodies were obtained with a size of 14 kDa by Western blot and demonstrated to have a good binding ability to PEDV SN protein.In summary,nanobodies with epitope fragments of PEDV S protein were suc-cessfully screened and prepared based on the phage display technology,which provided a new way for the in-depth application of nanobodies.
