Homocysteine aggravates cerebral ischemic injury in mice by monocyte chemoattractant protein-1
10.16557/j.cnki.1000-7547.2025.03.013
- VernacularTitle:同型半胱氨酸通过单核细胞趋化蛋白-1加重脑缺血小鼠损伤
- Author:
Wen HE
1
;
Yao WANG
1
;
Lizhou JIA
1
Author Information
1. 巴彦淖尔市医院神经内科,巴彦淖尔 015000
- Publication Type:Journal Article
- Keywords:
homocysteine(Hcy);
acute ischemic stroke;
monocyte chemotactic protein-1(MCP-1);
NF-κB;
mouse
- From:
Chinese Journal of Neuroanatomy
2025;41(3):359-367
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism by which homocysteine(Hcy)promotes the expression of mon-ocyte chemoattractant protein-1(MCP-1)and induces macrophage infiltration,exacerbating acute ischemic stroke(AIS).Methods:A focal cerebral ischemia-reperfusion mouse model was prepared using the suture method,and 2,3,5-triphenyl tetrazolium chloride(TTC)staining was used to detect the severity of cerebral ischemia,while HE staining was used to assess the extent of neuronal damage and inflammatory cell infiltration in ischemic brain tissue.High per-formance liquid chromatography(HPLC)was used to detect Hey expression in acute mouse brain tissue.Immunohisto-chemical staining,Western blot and real time RT-PCR were used to detect MCP-1 expression in mouse brain.Macro-phage migration was detected by Transwell chamber,and the effect of Hcy on endothelial cells was detected by TUNEL staining.The effect of MCP-1 neutralizing antibodies on macrophage migration was observed.Results:Compared with the sham group,the right cerebral hemisphere of the model group was significantly infarcted,ischemic brain tissue was severely damaged,nerve cells died and a large number of inflammatory cells infiltrated.The level of Hcy and the ex-pression of MCP-1 protein and mRNA were significantly increased.The treatment with Hcy can induce the nuclear translocation of p65,promote macrophage migration,and increase the endothelial cell apoptosis.Administration of p65 inhibitors reduced MCP-1 production.MCP-1 neutralizing antibodies significantly inhibited the macrophage migration.Conclusion:Hcy and MCP-1 is associated with the inflammatory response during cerebral ischemia-reperfusion,and Hcy may promote MCP-1 expression through NF-κB pathway,inducing macrophage infiltration and exacerbating cerebral ischemic injury.
