Protective effect of Tilianin on septicemia-induced myocardial injury in rat based on TLR4/NF-κB/NLRP3 signaling pathway
- VernacularTitle:基于TLR4/NF-κB/NLRP3信号通路探究田蓟苷对大鼠脓毒症心肌损伤的保护作用
- Author:
Jian-xin HAO
1
;
Yong-xin ZHANG
;
Yue WANG
;
Lei XU
;
Rui-fang ZHENG
;
Jian-guo XING
Author Information
- Publication Type:Journal Article
- Keywords: Tilianin; septicemia-induced myocardial injury; lipopolysaccharide; inflammation; NLRP3 in-flammasome; TLR4/NF-κB/NLRP3 signalling pathway
- From: Chinese Pharmacological Bulletin 2025;41(6):1135-1141
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the protective effects of Tilianin on lipopolysaccharide(LPS)-induced septice-mia-induced myocardial injury in rats and to explore the related mechanisms.Methods Animals were ran-domly grouped and a rat septicemia-induced myocardial injury model was constructed.Echocardiography was used to assess the cardiac function of rats,left ventricu-lar internal diameter at end-diastole(LVIDd)and left ventricular internal diameter at end-systole(LVIDs)were measured,and left ventricular ejection fraction(LVEF)and left ventricular fractional shortening(LVFS)were calculated;the kits were used to detect the serum activity of the relevant cardiac enzymes and the level of inflammatory factors;HE staining was used to observe the morphological changes of myocardium;immunofluorescence staining of cardiac tissues was used to detect the nuclear translocation of NF-κB p65;Western blot was used to detect the expression of TLR4,MyD88,p-NF-κB p65,and NLRP3 proteins in cardiac tissues.Results Compared with the model group,each administration group differently upregulated LVEF,LVFS,and LVIDs,and improved the coordina-tion of LV wall fluctuations in the model group of rats;cardiac enzymes LDH and CK-MB levels increased,and levels of inflammatory factors TNF-α,IL-6,and IL-1 β were reduced,exerting cardioprotective effects;HE staining showed that myocardial tissue cell gap was re-duced,myocardial fiber breakage was reduced,cardio-myocyte arrangement tended to be normal,and inflam-matory cell infiltration was reduced;NF-κB p65 entry into the nucleus was reduced,and phosphorylated NF-κB p65(p-NF-κB p65)expression was reduced;and Western blot results showed that the expression of TLR4,MyD88,and NLRP3 proteins was reduced.Conclusions Tilianin pretreatment reduces serum my-ocardial enzymes and inflammatory factors and im-proves myocardial injury in rats with septicemia-in-duced myocardial injury,which may be related to the Tilianin anti-TLR4/NF-κB/NLRP3 inflammatory signa-ling pathway.
