Endurance exercise alleviates cerebral ischemia/reperfusion injury in rats by activating Mas/PKA/CREB/UCP2 signaling pathway
- VernacularTitle:耐力运动通过激活Mas/PKA/CREB/UCP2信号通路减轻大鼠脑缺血/再灌注损伤
- Author:
Hui-sheng WU
1
;
Hua-xun WU
;
Wen-long DAI
;
Jun CHENG
;
Pei-pei GUO
Author Information
- Publication Type:Journal Article
- Keywords: endurance exercise; irisin; cerebral ische-mia; reperfusion injury; oxidative stress; Mas signa-ling pathway
- From: Chinese Pharmacological Bulletin 2025;41(6):1079-1085
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the effects of endur-ance exercise(EE)on rats following cerebral ischemi-a/reperfusion injury(CI/RI)and to explore its rela-tionship with the Mas signaling pathway.Methods Seventy-two adult male SD rats were randomly divided into four groups(n=18 each):sham group,model group,EE group(group E),and A779 pretreatment group(group A).The CI/RI model was established u-sing middle cerebral artery occlusion method Rats in both group E and group A underwent regular running for four weeks before model preparation,while rats in group A were injected with A779 30 minutes before model preparation.The cognitive function of rats was evaluated using the Neurological Disability Score(NDS)and Morris water maze test.After intravenous injection of Evans blue(EB)for one hour,the rats were euthanized,and brain tissues were collected to measure the infarction volume,EB content,ROS con-tent,and the percentage of apoptotic neurons of the hippocampal CA1 region.The protein expression relat-ed to the Mas pathway was detected by Western blot.Results Compared with the model group,the learning and memory ability as well as the neurological function in group E exhibited a significant improvement(P＜0.05).Both the cerebral infarction volume and the ap-optotic neuron percentage in the ischemic hippocampal CA1 region showed a significant reduction in group E(P＜0.05).The ROS content along with the EB con-tent in the brain tissue significantly decreased in group E(P＜0.05).Furthermore,the expressions of Mas/PKA/CREB/UCP2 pathway related proteins were sig-nificantly enhanced in group E(P＜0.05).However,the Mas receptor antagonist A779 significantly inhibited these neurological effects(P＜0.05).Conclusion EE may inhibit oxidative stress and alleviate CI/RI in rats by activating the Mas/PKA/CREB/UCP2 signaling pathway.
