Study on the mechanisms of " TongduAnshen " couplet medicines Notopterygii Rhizoma et Radix-Ziziphi Spinosae Semen on the improvement of depression-like behaviors in CUMS rats
10.16557/j.cnki.1000-7547.2025.03.008
- VernacularTitle:"通督安神"药对羌活-酸枣仁改善CUMS大鼠抑郁样行为的机制研究
- Author:
Yong ZHANG
1
;
Xinyue TANG
1
;
Wenxi CHENG
1
;
Chengpeng ZHANG
1
Author Information
1. 湖北中医药大学中医学院,武汉 430065
- Publication Type:Journal Article
- Keywords:
depression;
TongduAnshen;
hippocampus;
chronic unpredictable mild stress(CUMS);
couplet medi-cines;
rat
- From:
Chinese Journal of Neuroanatomy
2025;41(3):321-326
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanisms of " TongduAnshen " couplet medicines Notopterygii Rhizoma et Radix-Ziziphi Spinosae Semen(TDAS)on chronic unpredictable mild stress(CUMS)model rats.Methods:The rat model of depression were prepared using isolated and CUMS method.The depression-like behavior was detected by sucrose preference test(SPT),the open field test(OFT)and forced swimming test(FST).The pathological changes of hippocampal neurons were observed by HE and Nissl staining.The levels of 5-hydroxytryptamine(5-HT),interleukin 18(IL-18)and interleukin 1 β(IL-1β)were detected by enzyme linked immunosorbent assay(ELISA).The protein expression of NLRP3,ASC,caspase-1 in hippocampus were determined by Western blot.Results:The depression-like behavior of rats in the TDAS-L,TDAS-M and TDAS-H groups was significantly improved(P＜0.05 orP＜0.01);HE and Nissl staining showed that the neurons were arranged neatly,the number of shrunken cells were reduced,the extent of the injury was reduced;the contents of 5-HT in hippocampus of rats increased(P＜0.05 or P＜0.01),the contents of IL-18 and IL-1β in serum decreased(P＜0.01);the expressions of NLRP3,ASC and caspase-1 in hippocampus were decreased(P＜0.01).Conclusion:TDAS can improve depression-like behavior in chronic unpredictable mild stress rats,and its mechanism may be increase the contents of 5-HT,inhibit the expression of NLRP3 signaling pathway,and reduce the content of inflammatory factors.
