Mechanisms of polysaccharides from dendrobium officinale improving neurological function and sleep in ischemic stroke rats
10.16557/j.cnki.1000-7547.2025.03.006
- VernacularTitle:铁皮石斛多糖改善缺血性卒中大鼠神经功能及睡眠的机制研究
- Author:
Chunhua LI
1
;
Dingsheng MO
;
Kaimin ZENG
;
Jingjing PENG
;
Zhihong ZHAO
Author Information
1. 湖南省人民医院神经内科,长沙 410000
- Publication Type:Journal Article
- Keywords:
polysaccharides from dendrobium officinale;
ischemic stroke;
neurological function;
sleep;
oxidative stress;
autophagy;
pyroptosis;
rat
- From:
Chinese Journal of Neuroanatomy
2025;41(3):305-313
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects and mechanisms of dendrobium officinale polysaccharides(DOP)on neural function and sleep in a rat model of ischemic stroke.Methods:Rats with ischemic stroke were modeled using middle cerebral artery occlusion(MCAO).The neurological function was assessed using the Longa scale.Sleep was monitored by electroencephalography.The hippocampal tissue damage was evaluated by TTC staining,HE staining,Nissl staining and TUNEL staining.The kits were used to detect reactive oxygen species(ROS),malondialdehyde(MDA),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),IL-1β,and IL-18 in the hippocampal tissue.West-ern blot analysis was conducted to detect the expression of microtubule-associated protein light chain 3(LC3)and Bec-lin1,NLRP3,apoptosis-associated speck-like protein containing a caspase recruitment domain(ASC),cleaved caspase-1(C-caspase-1),GSDMD-N-terminal domain(GSDMD-N),nuclear factor erythroid 2-related factor 2(Nrf2),and heme oxygenase-1(HO-1)in the hippocampal tissue.Results:DOP can improve sleep quality and neurological func-tion in rats with ischemic stroke,alleviate hippocampal tissue damage,inhibit oxidative stress and inflammation in the hippocampus,decrease the expression of Beclin1,NLRP3,ASC,C-caspase-1,GSDMD-N,cytosolic Nrf2 protein,and the LC3-Ⅱ/LC3-Ⅰ ratio in the hippocampus,while increasing the expression of HO-1 and nuclear Nrf2 protein.The Nrf2 inhibitor ML385 partially reversed the ameliorative effects of DOP.Conclusion:DOP may improve neurological function and sleep in rats with ischemic stroke by inhibiting autophagy and NLRP3 inflammasome-mediated pyroptosis through the activation of the Nrf2/HO-1 pathway,thereby alleviating oxidative stress and neuroinflammation.
