Panax notoginseng saponin improves neurological function in cerebral ischemic rats by inhibiting AMPK/mTOR mediated autophagy
10.16557/j.cnki.1000-7547.2025.03.005
- VernacularTitle:三七总皂苷通过抑制AMPK/mTOR介导的自噬改善脑缺血大鼠神经功能
- Author:
Lu ZHNAG
1
;
Lijun YAN
1
Author Information
1. 重庆医药高等专科学校实训中心,重庆 400030
- Publication Type:Journal Article
- Keywords:
Panax notoginseng saponin(PNS);
ischemic stroke(IS);
autophagy;
neurological function;
AMPK/mTOR signaling pathway;
rat
- From:
Chinese Journal of Neuroanatomy
2025;41(3):298-304
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the neuroprotective effects of Panax notoginseng saponin(PNS)on rats with cere-bral ischemia.Method:Ninety-six Wistar rats were randomly divided into Sham operation(Sham)group,middle cere-bral artery occlusion(MCAO)group and MCAO+PNS group(intraperitoneal injection of PNS 100 mg/kg).Neurolog-ical function deficit score and balance beam test were used to evaluate neural and motor function in rats.2,3,5-triphe-nyl tetrazolium chloride(TTC)staining was used to detect the volume of cerebral infarction.Hematoxylin and eosin staining and Nissl staining were used to observe the pathological changes of hippocampal tissue.Levels of Beclin-1 and LC3-Ⅱ in the cerebral cortex of the rats were measured by immunohistochemical staining.The protein levels of Beclin-1,microtubule-associated protein 1 light chain 3-Ⅱ(LC3-Ⅱ)and AMP-activated protein kinase(AMPK)/mammalian target of rapamycin(mTOR)signaling pathway related factors in brain tissue were determined by Western blot.Results:Compared with Sham group,MCAO group showed neuronal necrosis,unclear cytoplasmic boundary and severe atrophy in the hippocampal tissue;Neurological deficit score,cross beam walking time and cerebral infarction volume were significantly increased(P＜0.05);Beclin-1 and LC3-Ⅱ protein expression levels,p-AMPKα/AMPKα ratio and the number of Beclin-1 and LC3-Ⅱ positive cells in brain tissue were significantly increased,while p-mTOR/mTOR ra-tio were significantly decreased(P＜0.05).After PNS treatment,the above phenomena were significantly improved.Conclusion:PNS can improve the neurological function of MCAO rats by inhibiting AMPK/mTOR pathway mediated autophagy,which is a potential effective drug for the treatment of cerebral ischemia.
