Exploring the Role of S100A16 in Endometrial Adenocarcinoma Tissues and Cells
10.11969/j.issn.1673-548X.2024.11.017
- VernacularTitle:探讨S100A16在子宫内膜腺癌组织及细胞中的作用
- Author:
Ying CAO
1
;
Jingbo ZHANG
;
Weiran ZHANG
Author Information
1. 221000 徐州医科大学研究生院
- Publication Type:Journal Article
- Keywords:
Endometrial neoplasms;
S100A16;
Proliferation;
Migration;
Invasion
- From:
Journal of Medical Research
2024;53(11):83-89
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of S100A16 in endometrial adenocarcinoma and its effect on the proliferation,migration and invasion ability of endometrial adenocarcinoma cells.Methods Immunohistochemistry was applied to detect the differences in the expression of S100A16 in endometrial adenocarcinoma and normal controls.According to the expression status,all endometrial ade-nocarcinoma patients were divided into S100A16 high-expression group and low-expression group,and then the differences in clinico-pathological characteristics of endometrial adenocarcinoma patients between the two groups were analyzed.S100A16 lentivirus was trans-fected into human endometrial adenocarcinoma cell lines Ishikawa and HEC-1A cells.CCK-8,Scratch assay and Transwell assay were used to detect the differences in proliferation,migration and invasive ability between the S100A16-shNC group and the S100A16-shR-NA group,respectively.Western blot was performed to detect the expression of proteins related to the epithelial mesenchymal transforma-tion(EMT)pathway protein expression.Results The expression of S100A16 in endometrial adenocarcinoma tissues was significantly higher than that in normal controls(P＜0.01).High expression of S100A16 was associated with worse pathological grading in patients with endometrial adenocarcinoma(P＜0.05),independent of age,International Federation of Gynecology and Obstetrics(FIGO)stage and lymph node metastasis aspects(P＞0.05).The results of CCK-8 assay showed that knockdown of S100A16 protein reduced the pro-liferation ability of endometrial adenocarcinoma cells(P＜0.01).Scratch assay and Transwell assay showed that the migration and inva-sion ability of endometrial adenocarcinoma cells in the S100A16-shNC group was higher than that in the S100A16-shRNA group(P＜0.05).Western blot revealed that E-cadherin expression was up-regulated,N-cadherin and Vimentin expression was down-regula-ted in endometrial adenocarcinoma cells in the S100A16-shRNA group compared with the S100A16-shNC group(P＜0.05).Conclusion S100A16 is highly expressed in endometrial adenocarcinoma tissues and promotes the malignant biological behaviour of en-dometrial adenocarcinoma cells by inducing EMT.
