Jiaotai Pill Ameliorates Type 2 Diabetes in Rats Via Modulation of Neurotransmitters in HPA-Related Tissues
10.11842/wst.20241119006
- VernacularTitle:交泰丸对T2DM大鼠神经递质系统紊乱的调控作用
- Author:
Xiaobin HUANG
1
;
Jianhua CHEN
;
Yanyi LI
;
Qing MIAO
;
Yujie ZHANG
Author Information
1. 北京中医药大学中药学院 北京 102844
- Publication Type:Journal Article
- Keywords:
Jiaotai Pill;
Type 2 diabetic rats;
Hypothalamic-pituitary-adrenal axis;
Neurotransmitters;
Mechanism of action
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2025;27(4):1047-1057
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effects of Jiaotai Pill(JTP)on type 2 diabetic(T2DM)rats,a systemic study was conducted by examining the changes in neurotransmitter levels related to the hypothalamic-pituitary-adrenal(HPA)axis.Methods The rats were divided into a normal group,a model group,a metformin group(183 mg·kg-1·d-1),and a JTP treatment group(3.3 g·kg-1·d-1).A T2DM model was established using a high-fat diet combined with streptozotocin.After 28 days of intragastric administration of JTP and metformin,metabolic indicators,Fasting blood glucose(FBG),insulin(INS),blood lipids,and pathological changes in the liver and kidneys were measured in each group of rats.Levels of eight neurotransmitters were quantified in serum,urine,and multiple tissues(brain,heart,liver,kidney,intestine,and adrenal gland)using ultra-high-performance liquid chromatography-tandem mass spectrometry.Results Compared to the T2DM model group,the JTP intervention groups showed varying degrees of reduction in water intake,food intake,FBG,INS,and blood lipids(P<0.05).Additionally,the fatty degeneration in the liver,glomerular lesions in the kidneys,and dyslipidemia were significantly improved.JTP intervention significantly modulated the disordered neurotransmitter levels in the blood,urine,and various tissues of T2DM rats(P<0.05),with the greatest number of neurotransmitters showing a notable recovery in blood,urine,brain,and adrenal gland.Conclusion JTP at a dosage of 3.3 g·kg-1·d-1 can improve the glucose and lipid metabolism in rats.JTP can regulate the HPA axis-related neurotransmitter system,mitigating metabolic disturbances and organ damage associated with T2DM.
